The Flow Cytometry Core Facility provides a broad range of applications to Rheumatic Disease Core CenterMembers including but not limited to the following: light scatter measurements assessing cell volume andstructural differences; single and multiple intracellular and/or surface immunofluorescence measurements;DNA ploidy measurements and cell cycle analysis; functional measurements of cellular calcium andpotassium flux and oxidative burst; detection and quantitation of apoptotic cell death, incident polarizationstudy; cell sorting on any or a combination of several analytical criteria.The Flow Cytometry Core Facility is currently located in the University of Michigan's Cancer and GeriatricsCenter's building and has been in continuous operation under the direction of Dr. Robert Todd since October1984. Investigators deliver pre-processed samples to the Core for flow cytometric analysis and/or cellsorting. Instruments are operated by trained flow cytometry operators (4.4 FTEs). Core instrumentationincludes a BD Biosciences FACSVantage SE multilaser high-speed cell sorter, two BD BiosciencesFACSDiVa multilaser high-speed cell sorters, a BD Biosciences FACSAria multilaser high-speed cell sorter,a BD Biosciences FACSCalibur multilaser analyzer and a Beckman-Coulter EPICS XL analyzer. UM-RDCC members (28 of 257) account for approximately 35% of total use of the core.
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