Glycosaminoglycan reactivity in lupus as a predictor of disease

Shih, Fei F.

Abstract

Funded Pilot and Feasibility Study #2: Title: Glycosaminoglycan reactivity in lupus as a predictor of disease PI: Fei Shih, M.D., Ph.D. A.
Specific Aims Systemic lupus erythematosus (SLE) is characterized by the production of autoantibodies and autoimmune attack of multiple organs. We hypothesized that there are novel targets yet to be discovered for SLE and these may serve as predictors of disease. Based on the highly patterned structure of DNA, we hypothesized that cross reactivity to glycosaminoglycans (GAGs), also a highly patterned structure, may serve as a biomarker for various disease manifestations. This pilot study examines aGAG reactivity in a murine model of SLE and extends aGAG analysis to pediatric patients with SLE with the following specific aims: 1) An unbiased examination of aGAG antibodies in KRN/G7m mice, a murine model of SLE;and 2) Characterize aGAG antibodies in SLE patients and correlation with disease.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
• Type: Center Core Grants (P30)
• Project #: 5P30AR048335-10
• Application #: 8130961
• Study Section: Special Emphasis Panel (ZAR1)
• Budget Start: 2010-09-01
• Budget End: 2011-08-31
• Support Year: 10
• Fiscal Year: 2010
• Total Cost: $89,657

Institution

• Name: Washington University
• DUNS #: 068552207
• City: Saint Louis
• State: MO
• Country: United States
• Zip Code: 63130

Publications

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Wilke, Georgia; Ravindran, Soumya; Funkhouser-Jones, Lisa et al. (2018) Monoclonal Antibodies to Intracellular Stages of Cryptosporidium parvum Define Life Cycle Progression In Vitro. mSphere 3:

Kulkarni, Hrishikesh S; Elvington, Michelle L; Perng, Yi-Chieh et al. (2018) Intracellular C3 Protects Human Airway Epithelial Cells from Stress-Associated Cell Death. Am J Respir Cell Mol Biol :

Garber, Charise; Vasek, Michael J; Vollmer, Lauren L et al. (2018) Astrocytes decrease adult neurogenesis during virus-induced memory dysfunction via IL-1. Nat Immunol 19:151-161

Yokoyama, Christine C; Baldridge, Megan T; Leung, Daisy W et al. (2018) LysMD3 is a type II membrane protein without an in vivo role in the response to a range of pathogens. J Biol Chem 293:6022-6038

Wu, Xiaobo; Hutson, Irina; Akk, Antonina M et al. (2018) Contribution of Adipose-Derived Factor D/Adipsin to Complement Alternative Pathway Activation: Lessons from Lipodystrophy. J Immunol 200:2786-2797

Kulkarni, Hrishikesh S; Liszewski, M Kathryn; Brody, Steven L et al. (2018) The complement system in the airway epithelium: An overlooked host defense mechanism and therapeutic target? J Allergy Clin Immunol 141:1582-1586.e1

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Roberson, Elisha D O (2018) Motif scraper: a cross-platform, open-source tool for identifying degenerate nucleotide motif matches in FASTA files. Bioinformatics 34:3926-3928

Knoop, Kathryn A; Gustafsson, Jenny K; McDonald, Keely G et al. (2017) Microbial antigen encounter during a preweaning interval is critical for tolerance to gut bacteria. Sci Immunol 2: