The High Throughput Cell Analysis Core is dedicated to the identification, functional and molecular characterization of muscle-derived cell populations of healthy and diseased adult skeletal muscle. This core includes prospective isolation of muscle-derived cell subpopulations by FACS (Muscle Sorting Service), a high throughput microscopy and screening of libraries of siRNAs, and microRNAs (mlRs) and chemical compounds (High Throughput Screening Service), and in vivo cell transplantation into mouse models of disease (Cell Transplantation Service). The flow cytometry, sorting, high throughput microscopy, chemical and functional genomics screening services are available in existing cores at SBMRI but not readily accessible to the non-SBMRI muscle community. This P30 will make these services available to the San Diego muscle research community. The High Throughput Cell Analysis Core will interface with the Phenotyping Core and the Imaging Core by assisting Center investigators with the appropriate isolation and characterization of muscle-derived cells from the mouse models or human tissues that are provided by the Center member. This core will make available to all Center investigators sophisticated flow-cytometry, high throughput imaging microscopy, and screening of noncoding RNAs (siRNA and mlRNA) and chemical compounds libraries. Moreover, the Core will also provide assistance and training for animal irradiation and cell transplantation. Additional services include single cell analysis and transplantation from Luciferase transgenic mice, enabling the monitoring the homing, proliferation and differentiation of transplanted cells in vivo.

Public Health Relevance

This Core will provide Center investigators with access to and training in isolation, functional analysis and characterization of muscle-derived cell populations sorted by a variety of high throughput analyses. The core will provide instruction on technology, data interpretation and troubleshooting of the proposed experiments. Overall, this Core will play an essential role in the understanding of the relative contribution of the different cell populations to skeletal muscle regeneration in physiological and pathological conditions.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
5P30AR061303-03
Application #
8532646
Study Section
Special Emphasis Panel (ZAR1-KM)
Project Start
Project End
Budget Start
2013-09-01
Budget End
2014-08-31
Support Year
3
Fiscal Year
2013
Total Cost
$193,652
Indirect Cost
Name
University of California San Diego
Department
Type
DUNS #
804355790
City
La Jolla
State
CA
Country
United States
Zip Code
92093
Madaro, Luca; Passafaro, Magda; Sala, David et al. (2018) Denervation-activated STAT3-IL-6 signalling in fibro-adipogenic progenitors promotes myofibres atrophy and fibrosis. Nat Cell Biol 20:917-927
Diez-Cuñado, Marta; Wei, Ke; Bushway, Paul J et al. (2018) miRNAs that Induce Human Cardiomyocyte Proliferation Converge on the Hippo Pathway. Cell Rep 23:2168-2174
Liu, Eva S; Martins, Janaina S; Zhang, Wanlin et al. (2018) Molecular analysis of enthesopathy in a mouse model of hypophosphatemic rickets. Development 145:
Sartorelli, Vittorio; Puri, Pier Lorenzo (2018) Shaping Gene Expression by Landscaping Chromatin Architecture: Lessons from a Master. Mol Cell 71:375-388
Marroncelli, Nicoletta; Bianchi, Marzia; Bertin, Marco et al. (2018) HDAC4 regulates satellite cell proliferation and differentiation by targeting P21 and Sharp1 genes. Sci Rep 8:3448
Gibbons, Michael C; Singh, Anshuman; Engler, Adam J et al. (2018) The role of mechanobiology in progression of rotator cuff muscle atrophy and degeneration. J Orthop Res 36:546-556
Gokhin, David S; Fowler, Velia M (2017) Software-based measurement of thin filament lengths: an open-source GUI for Distributed Deconvolution analysis of fluorescence images. J Microsc 265:11-20
Jordan, Sabine D; Kriebs, Anna; Vaughan, Megan et al. (2017) CRY1/2 Selectively Repress PPAR? and Limit Exercise Capacity. Cell Metab 26:243-255.e6
Roberts, Thomas C; Etxaniz, Usue; Dall'Agnese, Alessandra et al. (2017) BRD3 and BRD4 BET Bromodomain Proteins Differentially Regulate Skeletal Myogenesis. Sci Rep 7:6153
Latella, Lucia; Dall'Agnese, Alessandra; Boscolo, Francesca Sesillo et al. (2017) DNA damage signaling mediates the functional antagonism between replicative senescence and terminal muscle differentiation. Genes Dev 31:648-659

Showing the most recent 10 out of 86 publications