Abstract

The Flow Cytometry Shared Resource provides state-of-the-art cell sorting and analysis capability to the members of the Cancer Center. The Resource operates two cell sorters, a 4-color FacsVantage SE with a TurboSort option, and a newer FacsAria 6-color sorter with digital acquisition capabilities. These units are maintained and managed by dedicated operators. In addition, the Shared Resource maintains a total of three FacsCalibur analysis units; these 4-color machines are user-operated and provide a uniform platform to study cancer cells as well as the immune response to cancer. Capabilities include cell-cycle analysis, intracellular staining for cytokines and kinases, and cell-surface marker analysis. The cell-sorters are located in close proximity on the second floor of the Bunting Blaustein Cancer Research Building. This arrangement allows for two overlapping Resource managers to interdigitate services. The analysis units are user operated, and have been strategically placed throughout the Cancer Center to allow for optimal timely usage by a variety of individual researchers. Overall, this Core provides essential services for Cancer Center investigators from a diverse array of disciplines who seek to more deeply understand the biology and treatment of cancer.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA006973-46
Application #
7726519
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2008-05-01
Budget End
2009-04-30
Support Year
46
Fiscal Year
2008
Total Cost
$101,146
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Yuan, Ming; Da Silva, Ana Cristina A L; Arnold, Antje et al. (2018) MicroRNA (miR) 125b regulates cell growth and invasion in pediatric low grade glioma. Sci Rep 8:12506
Jacobs, Michael A; Macura, Katarzyna J; Zaheer, Atif et al. (2018) Multiparametric Whole-body MRI with Diffusion-weighted Imaging and ADC Mapping for the Identification of Visceral and Osseous Metastases From Solid Tumors. Acad Radiol 25:1405-1414
Annesley, Colleen E; Rabik, Cara; Duffield, Amy S et al. (2018) Knock-in of the Wt1 R394W mutation causes MDS and cooperates with Flt3/ITD to drive aggressive myeloid neoplasms in mice. Oncotarget 9:35313-35326
Liu, Tao; Ivaturi, Vijay; Sabato, Philip et al. (2018) Sorafenib Dose Recommendation in Acute Myeloid Leukemia Based on Exposure-FLT3 Relationship. Clin Transl Sci 11:435-443
Goodman, Melody; Lyons, Sarah; Dean, Lorraine T et al. (2018) How Segregation Makes Us Fat: Food Behaviors and Food Environment as Mediators of the Relationship Between Residential Segregation and Individual Body Mass Index. Front Public Health 6:92
Ramos, Juan C; Sparano, Joseph A; Rudek, Michelle A et al. (2018) Safety and Preliminary Efficacy of Vorinostat With R-EPOCH in High-risk HIV-associated Non-Hodgkin's Lymphoma (AMC-075). Clin Lymphoma Myeloma Leuk 18:180-190.e2
Wei, Ting; Najmi, Saman M; Liu, Hester et al. (2018) Small-Molecule Targeting of RNA Polymerase I Activates a Conserved Transcription Elongation Checkpoint. Cell Rep 23:404-414
Kasamon, Yvette L; Fuchs, Ephraim J; Zahurak, Marianna et al. (2018) Shortened-Duration Tacrolimus after Nonmyeloablative, HLA-Haploidentical Bone Marrow Transplantation. Biol Blood Marrow Transplant 24:1022-1028
Wang, Yuxuan; Li, Lu; Douville, Christopher et al. (2018) Evaluation of liquid from the Papanicolaou test and other liquid biopsies for the detection of endometrial and ovarian cancers. Sci Transl Med 10:
Walter, Vonn; Du, Ying; Danilova, Ludmila et al. (2018) MVisAGe Identifies Concordant and Discordant Genomic Alterations of Driver Genes in Squamous Tumors. Cancer Res 78:3375-3385

Showing the most recent 10 out of 2393 publications