Abstract

The Cancer Center Mass Spectrometry Core will identify and quantify proteins that change expression in well characterized protein fractions from cancerous cells or tissues. This will include identifying and quantifying changes in binding partners and post-translational modifications. Column chromatography and gel electrophoresis based one and two dimensional separations of protein complexes coupled to mass spectrometry will be used. Techniques such as difference gel electrophoresis (DIGE), isobaric tag for relative and absolute quantitation (iTRAQ) and 180-labeling will be available for quantify relative differences in protein expression. This Core will provide a senior staff technician to assist Center investigators and their staff in preparing and analyzing their samp

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA006973-48
Application #
8117679
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2010-05-01
Budget End
2011-04-30
Support Year
48
Fiscal Year
2010
Total Cost
$99,485
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Palsgrove, Doreen N; Brosnan-Cashman, Jacqueline A; Giannini, Caterina et al. (2018) Subependymal giant cell astrocytoma-like astrocytoma: a neoplasm with a distinct phenotype and frequent neurofibromatosis type-1-association. Mod Pathol 31:1787-1800
Adler, B L; Pezhouh, M K; Kim, A et al. (2018) Histopathological and immunophenotypic features of ipilimumab-associated colitis compared to ulcerative colitis. J Intern Med 283:568-577
Anchoori, Ravi K; Jiang, Rosie; Peng, Shiwen et al. (2018) Covalent Rpn13-Binding Inhibitors for the Treatment of Ovarian Cancer. ACS Omega 3:11917-11929
Shanbhag, Satish; Ambinder, Richard F (2018) Hodgkin lymphoma: A review and update on recent progress. CA Cancer J Clin 68:116-132
Cottrell, Tricia R; Duong, Anh T; Gocke, Christopher D et al. (2018) PD-L1 expression in inflammatory myofibroblastic tumors. Mod Pathol 31:1155-1163
Kelley, Dylan Z; Flam, Emily L; Guo, Theresa et al. (2018) Functional characterization of alternatively spliced GSN in head and neck squamous cell carcinoma. Transl Res 202:109-119
Knapp, Emily A; Dean, Lorraine T (2018) Consumer credit scores as a novel tool for identifying health in urban U.S. neighborhoods. Ann Epidemiol 28:724-729
Forde, Patrick M; Chaft, Jamie E; Smith, Kellie N et al. (2018) Neoadjuvant PD-1 Blockade in Resectable Lung Cancer. N Engl J Med 378:1976-1986
Paller, Channing J; Zhou, Xian C; Heath, Elisabeth I et al. (2018) Muscadine Grape Skin Extract (MPX) in Men with Biochemically Recurrent Prostate Cancer: A Randomized, Multicenter, Placebo-Controlled Clinical Trial. Clin Cancer Res 24:306-315
Fackler, Mary Jo; Sukumar, Saraswati (2018) Quantitation of DNA Methylation by Quantitative Multiplex Methylation-Specific PCR (QM-MSP) Assay. Methods Mol Biol 1708:473-496

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