Molecular Cytogenetics enables the analysis of chromosomal changes over a broad range of focus, fromwhole genome composition or organization to specific gene copy number or location. It provides acomprehensive genomic context for global or targeted cell biology studies. In contrast to most otherapproaches, it enables a cell-by-cell survey of chromosomal content, revealing heterogeneity and possibleassociations within that heterogeneity. Thus cytogenetic analysis remains a simple and efficient first steptowards identifying novel areas of genomic change.The Molecular Cytogenetics Core provides MSKCC investigators with effective chromosome-based analysesfor human or research animal cells. It processes samples from primary cells, cell lines, or archival tissue,performs chromosome analysis on research samples, using conventional Cytogenetics (chromosomebanding and karyotyping) and molecular Cytogenetics procedures based on fluorescence in situ hybridization(FISH), including Spectral Karyotyping (SKY). The Core staff works with investigators to design the mostappropriate and efficient analysis for their needs and produces customized probes for specific projects. TheCore has assembled a broad range of molecular Cytogenetics resources for human and mouse analysis,including plasmid and BAG clone stocks, as well as chromosome paints.Chromosome analysis is an integral part of research focusing on genomic instability. The Core's experiencein karyotyping and chromosome identification provides valuable support to investigators attempting tounderstand the basis of chromosomal instability in cancer.In addition to specific research applications, the Core also provides an essential function in maintainingGood Laboratory Practice for MSKCC research projects that use cultured cell lines. Karyotype analysisprovides basic confirmation and documentation of cell line identity, and is used to monitor chromosomalintegrity.
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