Molecular Cytogenetics enables the analysis of chromosomal changes over a broad range of focus, from whole genome composition or organization to specific gene copy number or location. It provides a comprehensive genomic context for global or targeted cell biology studies. In contrast to most other approaches, it enables a cell-by-cell survey of chromosomal content, revealing heterogeneity and possible associations within that heterogeneity. Thus cytogenetic analysis remains a simple and efficient first step towards identifying novel areas of genomic change. The Molecular Cytogenetics Core provides MSKCC investigators with effective chromosome-based analyses for human or research animal cells. It processes samples from primary cells, cell lines, or archival tissue, performs chromosome analysis on research samples, using conventional Cytogenetics (chromosome banding and karyotyping) and molecular Cytogenetics procedures based on fluorescence in situ hybridization (FISH), including Spectral Karyotyping (SKY). The Core staff works with investigators to design the most appropriate and efficient analysis for their needs and produces customized probes for specific projects. The Core has assembled a broad range of molecular Cytogenetics resources for human and mouse analysis, including plasmid and BAG clone stocks, as well as chromosome paints. Chromosome analysis is an integral part of research focusing on genomic instability. The Core's experience in karyotyping and chromosome identification provides valuable support to investigators attempting to understand the basis of chromosomal instability in cancer. In addition to specific research applications, the Core also provides an essential function in maintaining Good Laboratory Practice for MSKCC research projects that use cultured cell lines. Karyotype analysis provides basic confirmation and documentation of cell line identity, and is used to monitor chromosomal integrity.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA008748-46
Application #
8243719
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2011-01-01
Budget End
2011-12-31
Support Year
46
Fiscal Year
2011
Total Cost
$203,093
Indirect Cost
Name
Sloan-Kettering Institute for Cancer Research
Department
Type
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10065
Kavaler, Joshua; Duan, Hong; Aradhya, Rajaguru et al. (2018) miRNA suppression of a Notch repressor directs non-neuronal fate in Drosophila mechanosensory organs. J Cell Biol 217:571-583
Bosse, Tjalling; Nout, Remi A; McAlpine, Jessica N et al. (2018) Molecular Classification of Grade 3 Endometrioid Endometrial Cancers Identifies Distinct Prognostic Subgroups. Am J Surg Pathol 42:561-568
Hellmann, Matthew D; Nathanson, Tavi; Rizvi, Hira et al. (2018) Genomic Features of Response to Combination Immunotherapy in Patients with Advanced Non-Small-Cell Lung Cancer. Cancer Cell 33:843-852.e4
Scordo, Michael; Morjaria, Sejal M; Littmann, Eric R et al. (2018) Distinctive Infectious Complications in Patients with Central Nervous System Lymphoma Undergoing Thiotepa, Busulfan, and Cyclophosphamide-conditioned Autologous Stem Cell Transplantation. Biol Blood Marrow Transplant 24:1914-1919
Byron, Sara A; Tran, Nhan L; Halperin, Rebecca F et al. (2018) Prospective Feasibility Trial for Genomics-Informed Treatment in Recurrent and Progressive Glioblastoma. Clin Cancer Res 24:295-305
Zarnegar, Sara; Durham, Benjamin H; Khattar, Pallavi et al. (2018) Novel activating BRAF fusion identifies a recurrent alternative mechanism for ERK activation in pediatric Langerhans cell histiocytosis. Pediatr Blood Cancer 65:
Francis, Jasmine H; Slakter, Jason S; Abramson, David H et al. (2018) Treatment of juxtapapillary hemangioblastoma by intra-arterial (ophthalmic artery) chemotherapy with bevacizumab. Am J Ophthalmol Case Rep 11:49-51
Lee, Stanley Chun-Wei; North, Khrystyna; Kim, Eunhee et al. (2018) Synthetic Lethal and Convergent Biological Effects of Cancer-Associated Spliceosomal Gene Mutations. Cancer Cell 34:225-241.e8
Motzer, Robert J; Escudier, Bernard; Powles, Thomas et al. (2018) Long-term follow-up of overall survival for cabozantinib versus everolimus in advanced renal cell carcinoma. Br J Cancer 118:1176-1178
Giancipoli, Romina Grazia; Monti, Serena; Basturk, Olca et al. (2018) Complete metabolic response to therapy of hepatic epithelioid hemangioendothelioma evaluated with 18F-fluorodeoxyglucose positron emission tomography/contrast-enhanced computed tomography: A CARE case report. Medicine (Baltimore) 97:e12795

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