The Structural Biology Shared Resource provides the expertise needed to obtain high resolution structuresby NMR and X-ray diffraction. These structural approaches are complemented by significant strengths incomputational biology, cryo-electron microscopy, electron paramagnetic resonance, a wide range ofvibrational spectroscopies and the development of novel macromolecular footprinting approaches at theCollege and AECC. The X-ray component has access to ~50 days/year on the X9A bending magnetbeamline at the National Synchrotron Light Source (NSLS), Brookhaven National Laboratory. An additionalone day/month is currently available on the X29 insertion device at NSLS through the New York StructuralGenomics Research Consortium (NYSGRC), along with access to the X4 bending magnet beamlinesthrough Einstein's participation in the New York Structural Biology Center (NYSBC). The NYSGRC alsoprovides access to the SGX-CAT beamline at the Advanced Photon Source, which offers FEDEXcrystallographic service. These arrangements ensure that access to data collection resources is never ratelimiting. There is also in-house Rigaku rotating anode/image plate data collection capabilities essential forcharacterization and pre-screening of samples prior to synchrotron analysis. The NMR component at AECCconsists of 300 MHz and 600 MHz NMR spectrometers, one with a cryoprobe. In addition, AECCinvestigators have full access to the NYSBC which houses two new 900 MHz and three 800 MHzinstruments both with cryoprobes, and one 750 MHz solid state NMR spectrometer. The Facility is nowproviding dedicated protein expression and purification expertise initially on E. coli expression systems dueto the relative efficiency and throughput. It is anticipated that these efforts will be expanded to include higherorder expression systems (i.e., insect and mammalian cell systems) and cell free expression systems. Theenhancement of this service, as proposed in this application, is considered to be the single most direct routeto increasing the utilization of the Structural Biology Shared Resource by AECC members. The capability ofthe Facility has been strengthened by the acquisition of a custom designed TECAN crystallization robot thatcan support the rapid (minutes) generation of a 96-well crystallization trial.
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