A full Cancer Center-managed facility, the Radiation Research Shared Resource is located within andmaintained by the Center for Radiological Research. The facility provides a comprehensive irradiationservice for members of the HICCC primarily and others. The primary services provided are: X-ray irradiation of cells and animal models Gamma-ray irradiation of cells and animal models UV irradiation of cells and animal models Training of users in experimental design and safe operation of the irradiatorsDemand for the facility has steadily increased during the last six years, where the number of independentuser laboratories increased by 230% since 2000 (64 versus 28). The Radiation Shared Resource hasmachinery capable of exposing samples to x-rays, gamma-rays and ultraviolet light. Samples ranging fromsmall macromolecules such as DMA and proteins, to microorganisms, mammalian cells in culture or smallanimals can be exposed. Research goals include such tasks as creating double strand breaks or bulkylesions in DMA, irradiating mice before injection of cells to test tumorigenicity, creating transformed cells,preparing feeder layers for tissue culture, , and measuring radiosensitivity of yeast, mouse, hamster orhuman cells. The facility has four irradiators, a Siemans X-ray Therapy Unit, an Atomic Energy of CanadaGammacell 40 Cesium Unit, a high-dose-rate Gammacell 220 Cobalt Unit, and a UV light box emitting 254nm light. The operators of the facility provide users with guidance for safe and efficient sample exposure,as well as consultation on experimental design. The facility is operated in close association with theRadiation Safety Department to ensure compliance with governmental regulatory agencies. Future plansinclude the manufacture of an apparatus for subcellular stripe irradiation of cells in culture, anotherapparatus capable of rotating animals during x-ray exposure to permit irradiation of an internal anatomicaltarget while limiting exposure to surrounding tissues, and working with the Institute for ComparativeMedicine at CUMC to develop an imaging system for targeting radiation to anatomical sub-regions inmouse models of cancer.During the last period of the CCSG, 39% of the investigators using the facility were Cancer Centermembers with peer-reviewed funding with those members representing approximately 60% of the usage ofthe primary services. The proposed total operating budget of the facility is $110,022, of which we arerequesting $ 61,612 from the CCSG.
| Mumau, Melanie D; Vanderbeck, Ashley N; Lynch, Elizabeth D et al. (2018) Identification of a Multipotent Progenitor Population in the Spleen That Is Regulated by NR4A1. J Immunol 200:1078-1087 |
| Caviglia, Jorge Matias; Yan, Jun; Jang, Myoung-Kuk et al. (2018) MicroRNA-21 and Dicer are dispensable for hepatic stellate cell activation and the development of liver fibrosis. Hepatology 67:2414-2429 |
| Savage, Thomas M; Shonts, Brittany A; Obradovic, Aleksandar et al. (2018) Early expansion of donor-specific Tregs in tolerant kidney transplant recipients. JCI Insight 3: |
| Wu, Wen-Hsuan; Tsai, Yi-Ting; Justus, Sally et al. (2018) CRISPR Repair Reveals Causative Mutation in a Preclinical Model of Retinitis Pigmentosa: A Brief Methodology. Methods Mol Biol 1715:191-205 |
| Jauregui, Ruben; Park, Karen Sophia; Tsang, Stephen H (2018) Two-year progression analysis of RPE65 autosomal dominant retinitis pigmentosa. Ophthalmic Genet 39:544-549 |
| Ishida, Chiaki T; Zhang, Yiru; Bianchetti, Elena et al. (2018) Metabolic Reprogramming by Dual AKT/ERK Inhibition through Imipridones Elicits Unique Vulnerabilities in Glioblastoma. Clin Cancer Res 24:5392-5406 |
| Yen, Bonnie; Fortson, Katherine T; Rothman, Nyanza J et al. (2018) Clonal Bifurcation of Foxp3 Expression Visualized in Thymocytes and T Cells. Immunohorizons 2:119-128 |
| Renz, Bernhard W; Takahashi, Ryota; Tanaka, Takayuki et al. (2018) ?2 Adrenergic-Neurotrophin Feedforward Loop Promotes Pancreatic Cancer. Cancer Cell 33:75-90.e7 |
| Jin, Chun-Hui; Li, Yang; Xia, Jinxing et al. (2018) CXCR4 blockade improves leukemia eradication by allogeneic lymphocyte infusion. Am J Hematol 93:786-793 |
| Bakhoum, Mathieu F; Sengillo, Jesse D; Cui, Xuan et al. (2018) AUTOIMMUNE RETINOPATHY IN A PATIENT WITH A MISSENSE MUTATION IN PITPNM3. Retin Cases Brief Rep 12 Suppl 1:S72-S75 |
Showing the most recent 10 out of 331 publications
