Abstract

The University of Chicago Cancer Research Center DNA Sequencing Facility provides automated DNA sequencing services. The goals of the Facility are to provide accurate sequences at the lowest cost and the fastest turn- around time possible. The Facility includes an ABl 377 automated sequenator, 92-well and 48-well thermal cyclers, a Macintosh computer, a high resolution printer, and several software packages for sequence editing and alignment, including the latest version of Sequencer form Gene Codes. Users obtain order forms from the Facility World Wide Web page, fill them out (including the names and descriptions of each DNA template to be sequenced), place them in a personal file at the Facility, and then deliver a hard copy of the order together with the DNA samples. Facility technicians run the cycle sequencing reactions, usually dye- terminator reactions with primers supplied by the users, and then the sequencing gels, each with 36 lanes. The resulting chromatograms are placed in each user file, from which they can be down-loaded for editing and alignment. Turn-around time is 3-4 days. Routine results give good sequence for 600 bases; some templates can be read beyond 800. Facility staff provide users with advice on cloning vectors, template preparation, and planning of sequencing projects.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
3P30CA014599-26S1
Application #
6396720
Study Section
Project Start
1999-08-01
Project End
2000-07-31
Budget Start
Budget End
Support Year
26
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of Chicago
Department
Type
DUNS #
225410919
City
Chicago
State
IL
Country
United States
Zip Code
60637

  Publications

Trujillo, Jonathan A; Sweis, Randy F; Bao, Riyue et al. (2018) T Cell-Inflamed versus Non-T Cell-Inflamed Tumors: A Conceptual Framework for Cancer Immunotherapy Drug Development and Combination Therapy Selection. Cancer Immunol Res 6:990-1000
Zeng, Zongyue; Huang, Bo; Huang, Shifeng et al. (2018) The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs. Genes Dis 5:62-74
Lee, Ji-Hye; Park, Beom Seok; Han, Kang R et al. (2018) Insight Into the Interaction Between RNA Polymerase and VPg for Murine Norovirus Replication. Front Microbiol 9:1466
Cheng, Jason X; Chen, Li; Li, Yuan et al. (2018) RNA cytosine methylation and methyltransferases mediate chromatin organization and 5-azacytidine response and resistance in leukaemia. Nat Commun 9:1163
Johnson, Marianna B; Hoffmann, Joscelyn N; You, Hannah M et al. (2018) Psychosocial Stress Exposure Disrupts Mammary Gland Development. J Mammary Gland Biol Neoplasia 23:59-73
Sweis, Randy F; Zha, Yuanyuan; Pass, Lomax et al. (2018) Pseudoprogression manifesting as recurrent ascites with anti-PD-1 immunotherapy in urothelial bladder cancer. J Immunother Cancer 6:24
Kathayat, Rahul S; Cao, Yang; Elvira, Pablo D et al. (2018) Active and dynamic mitochondrial S-depalmitoylation revealed by targeted fluorescent probes. Nat Commun 9:334
Liu, Jun; Eckert, Mark A; Harada, Bryan T et al. (2018) m6A mRNA methylation regulates AKT activity to promote the proliferation and tumorigenicity of endometrial cancer. Nat Cell Biol 20:1074-1083
Bhanvadia, Raj R; VanOpstall, Calvin; Brechka, Hannah et al. (2018) MEIS1 and MEIS2 Expression and Prostate Cancer Progression: A Role For HOXB13 Binding Partners in Metastatic Disease. Clin Cancer Res 24:3668-3680
Wood, Kevin; Byron, Elizabeth; Janisch, Linda et al. (2018) Capecitabine and Celecoxib as a Promising Therapy for Thymic Neoplasms. Am J Clin Oncol 41:963-966

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