Abstract

The Electron Microscopy Facility provides a wide range of specialized services for electron microscopy and quantitative image analysis to members of the University of Chicago Cancer Research Center. Services range from negative stain analysis of single molecules and macromolecular assemblies, through transmission EM of cells and tissues, to scanning electron microscopy of embryos and whole mount specimens. The facility specializes in the immunogold localization of molecules, utilizing either frozen thin sectioning of aqueous samples, or freeze substitution of Lowicryl-embedded samples. The EM facility is equipped with both transmission and scanning electron microscopes, together with all the necessary ancillary equipment for the full range of EM services. In collaboration with the Digestive Disease Center EM facility operated by Dr. Robert Josephs, structural analysis of individual molecules is available using a cold transfer stage for viewing specimens at -170oC. Full time assistance is available for specimen preparation, microscopy, photographic imaging, quantitative image analysis and preparation of results for publication. Expert consultation is also available for project planning and data analysis. User services are coordinated with the UCCRC Digital Light Microscopy facility to provide integrated support for light and electron microscopy based research.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA014599-28
Application #
6592116
Study Section
Project Start
2002-05-15
Project End
2007-03-31
Budget Start
Budget End
Support Year
28
Fiscal Year
2002
Total Cost
Indirect Cost

  Institution

Name
University of Chicago
Department
Type
DUNS #
225410919
City
Chicago
State
IL
Country
United States
Zip Code
60637

  Publications

Trujillo, Jonathan A; Sweis, Randy F; Bao, Riyue et al. (2018) T Cell-Inflamed versus Non-T Cell-Inflamed Tumors: A Conceptual Framework for Cancer Immunotherapy Drug Development and Combination Therapy Selection. Cancer Immunol Res 6:990-1000
Zeng, Zongyue; Huang, Bo; Huang, Shifeng et al. (2018) The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs. Genes Dis 5:62-74
Lee, Ji-Hye; Park, Beom Seok; Han, Kang R et al. (2018) Insight Into the Interaction Between RNA Polymerase and VPg for Murine Norovirus Replication. Front Microbiol 9:1466
Cheng, Jason X; Chen, Li; Li, Yuan et al. (2018) RNA cytosine methylation and methyltransferases mediate chromatin organization and 5-azacytidine response and resistance in leukaemia. Nat Commun 9:1163
Johnson, Marianna B; Hoffmann, Joscelyn N; You, Hannah M et al. (2018) Psychosocial Stress Exposure Disrupts Mammary Gland Development. J Mammary Gland Biol Neoplasia 23:59-73
Sweis, Randy F; Zha, Yuanyuan; Pass, Lomax et al. (2018) Pseudoprogression manifesting as recurrent ascites with anti-PD-1 immunotherapy in urothelial bladder cancer. J Immunother Cancer 6:24
Kathayat, Rahul S; Cao, Yang; Elvira, Pablo D et al. (2018) Active and dynamic mitochondrial S-depalmitoylation revealed by targeted fluorescent probes. Nat Commun 9:334
Liu, Jun; Eckert, Mark A; Harada, Bryan T et al. (2018) m6A mRNA methylation regulates AKT activity to promote the proliferation and tumorigenicity of endometrial cancer. Nat Cell Biol 20:1074-1083
Bhanvadia, Raj R; VanOpstall, Calvin; Brechka, Hannah et al. (2018) MEIS1 and MEIS2 Expression and Prostate Cancer Progression: A Role For HOXB13 Binding Partners in Metastatic Disease. Clin Cancer Res 24:3668-3680
Wood, Kevin; Byron, Elizabeth; Janisch, Linda et al. (2018) Capecitabine and Celecoxib as a Promising Therapy for Thymic Neoplasms. Am J Clin Oncol 41:963-966

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