Abstract

The Antibody Development Facility provides investigators with custom designed monoclonal antibodies. In most, if not all cases, these are necessary reagents that are not commercially available. Services provided include: a) the design of antigens, immunization and screening strategies b) hybridoma cell line production via traditional methods, c) optimization of existing hybridoma cell lines by selection cloning d) production of monoclonal antibodies in species other than mice, e) services to wean hybridoma cell lines off selection media and into a variety of basal and serum free media for use in mini-bioreactors, immune precipitation or in vivo studies, f) cloning, expression and engineering of single chain antibody variable fragments (scFvs) or scFvs containing biological reporter molecules such as green fluorescent protein and g) the selection of antigen specific antibodies and their fragments from phage display libraries. This application requests continued support for a resource which continues to fulfill an essential role for peer reviewed research.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA015704-30
Application #
6728860
Study Section
Subcommittee G - Education (NCI)
Project Start
2003-03-12
Project End
2007-12-31
Budget Start
2003-03-12
Budget End
2003-12-31
Support Year
30
Fiscal Year
2003
Total Cost
$291,244
Indirect Cost

  Institution

Name
Fred Hutchinson Cancer Research Center
Department
Type
DUNS #
078200995
City
Seattle
State
WA
Country
United States
Zip Code
98109

  Publications

Walter, Roland B; Othus, Megan; Orlowski, Kaysey F et al. (2018) Unsatisfactory efficacy in randomized study of reduced-dose CPX-351 for medically less fit adults with newly diagnosed acute myeloid leukemia or other high-grade myeloid neoplasm. Haematologica 103:e106-e109
Thornblade, Lucas W; Mulligan, Michael S; Odem-Davis, Katherine et al. (2018) Challenges in Predicting Recurrence After Resection of Node-Negative Non-Small Cell Lung Cancer. Ann Thorac Surg 106:1460-1467
Adrianse, Robin L; Smith, Kaleb; Gatbonton-Schwager, Tonibelle et al. (2018) Perturbed maintenance of transcriptional repression on the inactive X-chromosome in the mouse brain after Xist deletion. Epigenetics Chromatin 11:50
Poston, Jacqueline N; Fromm, Jonathan R; Rasmussen, Heather A et al. (2018) A pilot study of weekly brentuximab vedotin in patients with CD30+ malignancies resistant to brentuximab vedotin every 3 weeks. Br J Haematol :
Lee, Dale; Swan, C Kaiulani; Suskind, David et al. (2018) Children with Crohn's Disease Frequently Consume Select Food Additives. Dig Dis Sci 63:2722-2728
Nambiar, Mridula; Smith, Gerald R (2018) Pericentromere-Specific Cohesin Complex Prevents Meiotic Pericentric DNA Double-Strand Breaks and Lethal Crossovers. Mol Cell 71:540-553.e4
Duggan, Catherine; Tapsoba, Jean de Dieu; Stanczyk, Frank et al. (2018) Long-term weight loss maintenance, sex steroid hormones, and sex hormone-binding globulin. Menopause :
Yu, Ming; Maden, Sean K; Stachler, Matthew et al. (2018) Subtypes of Barrett's oesophagus and oesophageal adenocarcinoma based on genome-wide methylation analysis. Gut :
Georges, George E; Doney, Kris; Storb, Rainer (2018) Severe aplastic anemia: allogeneic bone marrow transplantation as first-line treatment. Blood Adv 2:2020-2028
Fowler, Kyle R; Hyppa, Randy W; Cromie, Gareth A et al. (2018) Physical basis for long-distance communication along meiotic chromosomes. Proc Natl Acad Sci U S A 115:E9333-E9342

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