Abstract

The Antibody Development Facility provides Fred Hutchinson/University of Washington Cancer Consortium (Consortium) investigators with custom designed monoclonal and recombinant (scFv) antibodies. Intact monoclonal antibodies are produced via traditional hybridoma technology while recombinant antibodies are produced via phage display technology. Recombinant antibody fragments containing Vh and VI chains (scFvs) are derived by bio panning on the relevant antigen via both positive and negative panning techniques. Use of both of these technologies has permitted the identification antibodies useful for traditional biochemical, cell biological and immunological characterization of protein antigens, the creation of DNA vaccines, as well as high through put technologies useful for identification of antibodies directed to nonimmunogenic peptide antigens as well as biomarker discovery (scFv antibody arrays). This application requests continued support for a resource which continues to fulfill an essential role for research within the Consortium.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA015704-39
Application #
8561928
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2013-01-01
Budget End
2013-12-31
Support Year
39
Fiscal Year
2013
Total Cost
$129,531
Indirect Cost
$49,409

  Institution

Name
Fred Hutchinson Cancer Research Center
Department
Type
DUNS #
078200995
City
Seattle
State
WA
Country
United States
Zip Code
98109

  Publications

Mora-Pinzon, Maria C; Trentham-Dietz, Amy; Gangnon, Ronald E et al. (2018) Urinary Magnesium and Other Elements in Relation to Mammographic Breast Density, a Measure of Breast Cancer Risk. Nutr Cancer 70:441-446
Shirts, Brian H; Konnick, Eric Q; Upham, Sarah et al. (2018) Using Somatic Mutations from Tumors to Classify Variants in Mismatch Repair Genes. Am J Hum Genet 103:19-29
Du, Jing; Flynn, Ryan; Paz, Katelyn et al. (2018) Murine chronic graft-versus-host disease proteome profiling discovers CCL15 as a novel biomarker in patients. Blood 131:1743-1754
Verboon, Jeffrey M; Decker, Jacob R; Nakamura, Mitsutoshi et al. (2018) Wash exhibits context-dependent phenotypes and, along with the WASH regulatory complex, regulates Drosophila oogenesis. J Cell Sci 131:
Kuzma, Jessica N; Hagman, Derek K; Cromer, Gail et al. (2018) Intra-individual variation in markers of intestinal permeability and adipose tissue inflammation in healthy normal weight to obese adults. Cancer Epidemiol Biomarkers Prev :
Suzuki, Aussie; Gupta, Amitabha; Long, Sarah K et al. (2018) A Kinesin-5, Cin8, Recruits Protein Phosphatase 1 to Kinetochores and Regulates Chromosome Segregation. Curr Biol 28:2697-2704.e3
Walter, Roland B; Othus, Megan; Orlowski, Kaysey F et al. (2018) Unsatisfactory efficacy in randomized study of reduced-dose CPX-351 for medically less fit adults with newly diagnosed acute myeloid leukemia or other high-grade myeloid neoplasm. Haematologica 103:e106-e109
Thornblade, Lucas W; Mulligan, Michael S; Odem-Davis, Katherine et al. (2018) Challenges in Predicting Recurrence After Resection of Node-Negative Non-Small Cell Lung Cancer. Ann Thorac Surg 106:1460-1467
Adrianse, Robin L; Smith, Kaleb; Gatbonton-Schwager, Tonibelle et al. (2018) Perturbed maintenance of transcriptional repression on the inactive X-chromosome in the mouse brain after Xist deletion. Epigenetics Chromatin 11:50
Poston, Jacqueline N; Fromm, Jonathan R; Rasmussen, Heather A et al. (2018) A pilot study of weekly brentuximab vedotin in patients with CD30+ malignancies resistant to brentuximab vedotin every 3 weeks. Br J Haematol :

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