Abstract

The Immune Monitoring Shared Resource provides investigators with a broad range of services and expertise to perform human protein and other biological mediator analyses and to provide precise evaluation of immunologic responses for both research and clinical studies. Resource services include the production of peptide-major histocompatibility complex Class-I tetramers, reagents useful for accessing antigen-specific T cells; molecular assays for interrogating T cell receptor usage and frequency, cellular assays for determining cell function, and high throughput analytical services using ELISA assay and Luminex microbead methodology. Support is provided for both single experiments as well as large long-term clinical studies. The IMSR works closely with the Therapeutic Manufacturing Shared Resource to support clinical trials. Instrumentation includes two fast protein liquid chromatography (FPLC) systems, multi-mode hybrid reader, ELISPOT reader, enzyme-linked immunosorbent assay (ELISA) reader, xCELLigence System, luminometer, two quantitative PCR (qPCR) systems, a robotic liquid handling system, a Top-Count scintillation counter and two Luminex 200 systems.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA015704-44
Application #
9617710
Study Section
Subcommittee I - Transistion to Independence (NCI)
Project Start
Project End
Budget Start
2019-01-01
Budget End
2019-12-31
Support Year
44
Fiscal Year
2019
Total Cost
Indirect Cost

  Institution

Name
Fred Hutchinson Cancer Research Center
Department
Type
DUNS #
078200995
City
Seattle
State
WA
Country
United States
Zip Code
98109

  Publications

Buckley, Sarah A; Percival, Mary-Elizabeth; Othus, Megan et al. (2018) A comparison of patients with acute myeloid leukemia and high-risk myelodysplastic syndrome treated on versus off study. Leuk Lymphoma :1-7
Montgomery, Elizabeth T; Noguchi, Lisa M; Dai, James Y et al. (2018) Acceptability of and Adherence to an Antiretroviral-Based Vaginal Microbicide among Pregnant Women in the United States. AIDS Behav 22:402-411
Talarico, Sarah; Leverich, Christina K; Wei, Bing et al. (2018) Increased H. pylori stool shedding and EPIYA-D cagA alleles are associated with gastric cancer in an East Asian hospital. PLoS One 13:e0202925
Rañola, John Michael O; Liu, Quanhui; Rosenthal, Elisabeth A et al. (2018) A comparison of cosegregation analysis methods for the clinical setting. Fam Cancer 17:295-302
Greenbaum, Adam M; Green, Damian J; Holmberg, Leona A et al. (2018) Bendamustine, etoposide, and dexamethasone to mobilize peripheral blood hematopoietic stem cells for autologous transplantation in non-Hodgkin lymphoma. Blood Res 53:223-226
Zhao, Shanshan; Leonardson, Amy; Geybels, Milan S et al. (2018) A five-CpG DNA methylation score to predict metastatic-lethal outcomes in men treated with radical prostatectomy for localized prostate cancer. Prostate :
Giraldo, Nicolas A; Nguyen, Peter; Engle, Elizabeth L et al. (2018) Multidimensional, quantitative assessment of PD-1/PD-L1 expression in patients with Merkel cell carcinoma and association with response to pembrolizumab. J Immunother Cancer 6:99
DeWitt 3rd, William S; Smith, Anajane; Schoch, Gary et al. (2018) Human T cell receptor occurrence patterns encode immune history, genetic background, and receptor specificity. Elife 7:
Herman, Daniel S; Smith, Christina; Liu, Chang et al. (2018) Efficient Detection of Copy Number Mutations in PMS2 Exons with a Close Homolog. J Mol Diagn 20:512-521
Birnbaum, Jeanette K; Duggan, Catherine; Anderson, Benjamin O et al. (2018) Early detection and treatment strategies for breast cancer in low-income and upper middle-income countries: a modelling study. Lancet Glob Health 6:e885-e893

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