The Flow Cytometry and Cell Sorting Facility was established by the Abramson Cancer Center in 1980, and has been continuously approved and funded by the NCI Cancer Center Support Grant since 1981. In the last competitive renewal, this Shared Resource received an ?exceptional? merit evaluation. The Facility is recognized as one of the largest and most comprehensive academic flow cytometry shared resources in the United States, offering access to a broad range of analytical and sorting cytometers, expert consultation, and an extensive educational program. Jonni Moore, PhD, Professor of Pathology and Laboratory Medicine, has directed the Facility since 1990. She has a doctorate in immunology and is a noted worldwide expert in both the application of high dimensional flow cytometry and shared resource operation. The technical staff is managed by Charles Pletcher, MCI, who has been with the Facility since 1987. He brings over 25 years of extensive technical instrumentation and application experience to the Facility. The staff of the Facility is comprised of experienced technologists, each with an average of 12 years experience, who offer ACC members access to cutting edge cytometric technology, as well as expert support in experimental design and data evaluation. During the current project period, the Flow Cytometry and Cell Sorting Facility remained one of the most frequently used CCSG-funded Shared Resources of the Abramson Cancer Center, and continued to experience growth. ACC members accounted for 110 of the 265 researchers (41%) who used the Facility during the reporting period (10/01/13-09/30/14), the vast majority of whom (102/110) have peer-reviewed funding. CCSG support represents 10% of the proposed Facility budget with the remaining funding coming from charge backs, additional grant support and Institutional support. The success of the Shared Resource during the project period has been facilitated by the addition of instrumentation, recruitment of staff, expansion of training programs and the increased interest in cytomics within the ACC membership.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA016520-40
Application #
8998399
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2015-12-01
Budget End
2016-11-30
Support Year
40
Fiscal Year
2016
Total Cost
$151,594
Indirect Cost
$56,848
Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Huang, Mo; Wang, Jingshu; Torre, Eduardo et al. (2018) SAVER: gene expression recovery for single-cell RNA sequencing. Nat Methods 15:539-542
Yam, Clinton; Xu, Xiaowei; Davies, Michael A et al. (2018) A Multicenter Phase I Study Evaluating Dual PI3K and BRAF Inhibition with PX-866 and Vemurafenib in Patients with Advanced BRAF V600-Mutant Solid Tumors. Clin Cancer Res 24:22-32
Onorati, Angelique V; Dyczynski, Matheus; Ojha, Rani et al. (2018) Targeting autophagy in cancer. Cancer 124:3307-3318
Rebecca, Vito W; Nicastri, Michael C; Fennelly, Colin et al. (2018) PPT1 promotes tumor growth and is the molecular target of chloroquine derivatives in cancer. Cancer Discov :
Garfall, Alfred L; Stadtmauer, Edward A; Hwang, Wei-Ting et al. (2018) Anti-CD19 CAR T cells with high-dose melphalan and autologous stem cell transplantation for refractory multiple myeloma. JCI Insight 3:
Jang, Jeong Hoon; Manatunga, Amita K; Taylor, Andrew T et al. (2018) Overall indices for assessing agreement among multiple raters. Stat Med 37:4200-4215
Scheel, John R; Kim, Eunhee; Partridge, Savannah C et al. (2018) MRI, Clinical Examination, and Mammography for Preoperative Assessment of Residual Disease and Pathologic Complete Response After Neoadjuvant Chemotherapy for Breast Cancer: ACRIN 6657 Trial. AJR Am J Roentgenol 210:1376-1385
Romero, Sally A D; Brown, Justin C; Bauml, Joshua M et al. (2018) Barriers to physical activity: a study of academic and community cancer survivors with pain. J Cancer Surviv 12:744-752
Hinderer, Christian; Katz, Nathan; Buza, Elizabeth L et al. (2018) Severe Toxicity in Nonhuman Primates and Piglets Following High-Dose Intravenous Administration of an Adeno-Associated Virus Vector Expressing Human SMN. Hum Gene Ther 29:285-298
Li, Jinyang; Byrne, Katelyn T; Yan, Fangxue et al. (2018) Tumor Cell-Intrinsic Factors Underlie Heterogeneity of Immune Cell Infiltration and Response to Immunotherapy. Immunity 49:178-193.e7

Showing the most recent 10 out of 1047 publications