Pharmacology and Analytical Facility - HPLC and Mass Spectrometry FacilityThis facility provides access to sophisticated HPLC, ultra high performance liquid chromatographic and massspectrometry instruments. In this facility, analytical assay methodologies can be developed and validatedusing UPLC/HPLC equipment with a broad array of quantitative detection systems (e.g. UV, photodiodearray, fluorescence, radiometric, light scattering, electrochemical). Current mass spectrometry instrumentsinclude a simple single quad LC/MS instrument for use in molecular weight determinations as well as morecomplex tandem mass spectrometers (7 total), including a time-of-flight instrument for more intricate drugmetabolism studies and experiments requiring accurate mass weight measurements. In addition, a GC/MSinstrument is available for separation and quantitation of volatile compounds. Recently added wereautomated solid phase and liquid extraction platforms capable of isolating multiple analytes of interest(drugs, macromolecules, and protein drug targets) from complex biomatrices (blood, plasma, normal organtissues, and tumors). These extraction systems have improved both extraction efficiency and throughput inanalytical sample preparation. The laboratory has supported experiments in chemical synthesis, drug libraryscreening, medicinal chemistry, drug discovery and development, drug metabolism, drug target identification,drug metabolism, drug tissue biodistribution, systemic pharmacokinetics, drug production solubility andstability, formulation, and tumor biochemistry. The HPLC/Mass Spectrometry Facility sampled analysisincreased over 340% since 1999 and is now analyzing more than 33,000 samples per year serving over 52faculty members who are members of 21 CCSG Programs; 90% of the users have peer-reviewed funding.The current source of funding is 48.4% CCSG, and 51.6% user fees. This state of the art facility is located ata newly renovated El Rio campus located approximately 1 mile from the main campus. This location is a7250 sq. ft. facility that houses 4 faculty members and 21 support staff as well as 25 full UPLC/HPLCsystems. In addition to the advanced analytical facility the combined program faculty have in excess of 60years of experience in analytical chemistry, analytical method development and validation, isolationtechnologies, pharmacology, drug metabolism, and pharmacokinetic/pharmacodynamics. They act as aninvaluable resource in experimental design, development and implementation to the user faculty who oftenlack the specific training to conduct experiments requiring quantitative analytical chemistry andpharmacology.
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