The mission of the Cell Biology & Microinjection component of Reproductive Sciences is to provide CancerCenter members with access to centralized facilities, instrumentation and technical expertise necessary toallow investigators to carry out embryonic stem (ES) cell line derivation, electroporation, and to creategenetically modified mice for in vivo studies of gene function. The Service generates genetically modifiedmice on a variety of inbred strain and hybrid backgrounds and has derived germline-competent ES celllines from inbred mouse strains. Germline-competence validation, training, custom projects, and testedreagents and supplies are also offered. The Service, which has previously been supported by CancerCenter support grant (CCSG) funds, works closely with Cryopreservation, Importation and Rederivationwithin Reproductive Sciences, as well as Phenotyping Sciences to provide Cancer Center members wellintegrated comprehensive services. In 2004 the Cell Biology & Microinjection Service merged with theReproductive Technologies Resource to form Reproductive Sciences. The synergy created by the mergerallowed management and staff to identify complementary and overlapping functions within the groups;establish new collaborative service offerings; merge similar processes to eliminate redundancy; andcombine laboratory, vivarium and office space to maximize productivity. Cell Biology & MicroinjectionService personnel work closely with other members of Reproductive Sciences and the Molecular BiologyService to offer Cancer Center investigators seamless access to services. This close internal collaborationinsures that investigators with expertise outside of these areas can fully employ current transgenic andgene targeting technology in their own work by utilizing these interactive services. Senior Staff Scientist Dr.Thomas Gridley was named Faculty Advisor (Project Leader) of Cell Biology & Microinjection Service in1999. He oversees this fee-for-service operation which employs two ES cell technologists, twomicroinjection technologists, a colony manager and senior operational manager. The Service occupies1,899 ft2 of laboratory, vivarium and office space in Research Laboratory Building 4 and the GeneticsResources Building. Dr. Gridley communicates with the Cancer Center users, Service staff and CenterAdministration to ensure that research needs are met in the most efficient, cost-effective and technicallycurrent manner.
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