The Advanced Microscopy Facility was established in 1979 and has, since the first Cancer Center Support Grant to the University of Virginia in 1987, provided Cancer Center investigators with unique access to instrumentation and services for critical investigations regarding the cause, spread, and treatment of cancer Access to cutting edge microscopy equipment at the AMF has allowed Cancer Center members specifically to elucidate the role of PK1 in cytokinesis, to identify a role for the tumor suppressor APC in mRNA localization, to further define the role of SCAMPS in regulation of cell proliferation, and to identify some of the factors involved in the organization and orientation of epithelial cells, all of which are critical cell functions that play pivotal roles in the development and spread of cancer. The facility, which is both a full-service and a user-facility, has been expanded from primarily an electron microscopy center to encompass a full range of light and electron microscopy technologies. This expansion includes the acquisition of four confocal microscopes and one transmission electron microscope, and relocation of the facility to renovated quarters, doubling the previous laboratory space. The primary objective of the facility is to provide investigators access to, and assistance with, state-of-the art confocal and electron microscopes, preparatory equipment, and applications. An ongoing emphasis of this Shared Resource is to provide facilities for the manipulation and imaging of live cells, and to track the dynamics of fluorophores in cells under physiological conditions. Within this overall framework, related objectives include: (1) providing consultation on research objectives which employ electron and light microscopy methods;(2) assuring that up-to-date equipment is available and properly maintained for use by investigators;(3) providing complete electron microscopy specimen preparation services;(4) educating and training clients in the use of equipment or specialized techniques;and (5) monitoring developments in the field of microscopy that might be incorporated into the services offered by the facility.
| Manukyan, Arkadi; Kowalczyk, Izabela; Melhuish, Tiffany A et al. (2018) Analysis of transcriptional activity by the Myt1 and Myt1l transcription factors. J Cell Biochem 119:4644-4655 |
| Engelhard, Victor H; Rodriguez, Anthony B; Mauldin, Ileana S et al. (2018) Immune Cell Infiltration and Tertiary Lymphoid Structures as Determinants of Antitumor Immunity. J Immunol 200:432-442 |
| Martins, André L; Walavalkar, Ninad M; Anderson, Warren D et al. (2018) Universal correction of enzymatic sequence bias reveals molecular signatures of protein/DNA interactions. Nucleic Acids Res 46:e9 |
| Michaels, Alex D; Newhook, Timothy E; Adair, Sara J et al. (2018) CD47 Blockade as an Adjuvant Immunotherapy for Resectable Pancreatic Cancer. Clin Cancer Res 24:1415-1425 |
| Shi, Lei; Li, Kang; Guo, Yizhan et al. (2018) Modulation of NKG2D, NKp46, and Ly49C/I facilitates natural killer cell-mediated control of lung cancer. Proc Natl Acad Sci U S A 115:11808-11813 |
| Yang, Jun; LeBlanc, Francis R; Dighe, Shubha A et al. (2018) TRAIL mediates and sustains constitutive NF-?B activation in LGL leukemia. Blood 131:2803-2815 |
| Kulling, Paige M; Olson, Kristine C; Hamele, Cait E et al. (2018) Dysregulation of the IFN-?-STAT1 signaling pathway in a cell line model of large granular lymphocyte leukemia. PLoS One 13:e0193429 |
| Grant, Margaret J; Loftus, Matthew S; Stoja, Aiola P et al. (2018) Superresolution microscopy reveals structural mechanisms driving the nanoarchitecture of a viral chromatin tether. Proc Natl Acad Sci U S A 115:4992-4997 |
| Knapp, Kiley A; Pires, Eusebio S; Adair, Sara J et al. (2018) Evaluation of SAS1B as a target for antibody-drug conjugate therapy in the treatment of pancreatic cancer. Oncotarget 9:8972-8984 |
| Kedzierska, Katarzyna Z; Gerber, Livia; Cagnazzi, Daniele et al. (2018) SONiCS: PCR stutter noise correction in genome-scale microsatellites. Bioinformatics 34:4115-4117 |
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