Abstract

The Flow Cytometry (FACS) Shared Resource assists Cancer Center members in performing cell sorting and fluorescence quantification using flow cytometry. The Shared Resource consists of two flow cytometers-a Coulter Epics Elite ESP flow cytometer/cell sorter and an automated benchtop Becton Dickinson FACS Caliber flow cytometer-and a full time technician. It is supervised by Dr. Scott Lowe (Associate Professor) and Ms. Linda Rogers (Senior Research Associate), and is operated by Ms. Klara Velinzon (Research Technician). The Facility is used by a large number of Cancer Center investigators for a variety of research procedures, ranging from sorting of specific cell types within complex populations to more routine fluorescence quantification. The research assistance provided by the FACS Shared Resource is essential for the cancer research conducted in the CSHL Cancer Center, and helps to integrate our research programs. We continue to request support for fixed costs of this faculty. Because fixed, central costs are a large population of the total costs, the Cancer Center Support Grant is absolutely essential for this Shared Resource and for the research it allows.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA045508-14
Application #
6348963
Study Section
Project Start
2000-09-08
Project End
2001-07-31
Budget Start
Budget End
Support Year
14
Fiscal Year
2000
Total Cost
Indirect Cost

  Institution

Name
Cold Spring Harbor Laboratory
Department
Type
DUNS #
065968786
City
Cold Spring Harbor
State
NY
Country
United States
Zip Code
11724

  Publications

Knott, Simon R V; Wagenblast, Elvin; Khan, Showkhin et al. (2018) Asparagine bioavailability governs metastasis in a model of breast cancer. Nature 554:378-381
Biffi, Giulia; Oni, Tobiloba E; Spielman, Benjamin et al. (2018) IL-1-induced JAK/STAT signaling is antagonized by TGF-beta to shape CAF heterogeneity in pancreatic ductal adenocarcinoma. Cancer Discov :
Xie, Yuanyuan; Cao, Zhen; Wong, Elissa Wp et al. (2018) COP1/DET1/ETS axis regulates ERK transcriptome and sensitivity to MAPK inhibitors. J Clin Invest 128:1442-1457
Aznarez, Isabel; Nomakuchi, Tomoki T; Tetenbaum-Novatt, Jaclyn et al. (2018) Mechanism of Nonsense-Mediated mRNA Decay Stimulation by Splicing Factor SRSF1. Cell Rep 23:2186-2198
Fang, Han; Huang, Yi-Fei; Radhakrishnan, Aditya et al. (2018) Scikit-ribo Enables Accurate Estimation and Robust Modeling of Translation Dynamics at Codon Resolution. Cell Syst 6:180-191.e4
Lin, Kuan-Ting; Ma, Wai Kit; Scharner, Juergen et al. (2018) A human-specific switch of alternatively spliced AFMID isoforms contributes to TP53 mutations and tumor recurrence in hepatocellular carcinoma. Genome Res :
Wolff, Robert A; Wang-Gillam, Andrea; Alvarez, Hector et al. (2018) Dynamic changes during the treatment of pancreatic cancer. Oncotarget 9:14764-14790
Ryan, Niamh M; Lihm, Jayon; Kramer, Melissa et al. (2018) DNA sequence-level analyses reveal potential phenotypic modifiers in a large family with psychiatric disorders. Mol Psychiatry 23:2254-2265
Danko, Charles G; Choate, Lauren A; Marks, Brooke A et al. (2018) Dynamic evolution of regulatory element ensembles in primate CD4+ T cells. Nat Ecol Evol 2:537-548
Ahrens, Sandra; Wu, Melody V; Furlan, Alessandro et al. (2018) A Central Extended Amygdala Circuit That Modulates Anxiety. J Neurosci 38:5567-5583

Showing the most recent 10 out of 380 publications