The major goal of the Protein Chemistry Resource is to provide sequence information of isolated proteins in low abundance at the Cancer Center of Cold Spring Harbor Laboratory. Sequence information is used to isolate genes or identify the genes in the database. Another goal is to analyze post-transcriptional modification of proteins to study protein function, protein-protein interaction and protein-nucleic acid interaction in cancer research. The Resource provides: protein sequence analysis by in-gel digestion, HPLC peptide mapping and automated Edman degradation N-terminal sequencing of protein blotted on PVDF membrane post-translational modification analysis by MALDI-TOF-mass spectrometry and HPLC peptide mapping followed by automated Edman degradation epitope mapping by using mass spectrometry, HPLC and peptide sequencer molecular weight determination of peptides, proteins, and other molecules mass spectrometry sequencing y LCQ deca electrospray mass spectrometry including post-translation modification site analysis Since there are so many proteins isolated with biological significance in all areas of molecular biology, genetics, and cell biology at the Cancer Center, the Protein Chemistry Shared Resource is used by a large number of the Cancer Center at Cold Spring Harbor Laboratory
| Aberle, M R; Burkhart, R A; Tiriac, H et al. (2018) Patient-derived organoid models help define personalized management of gastrointestinal cancer. Br J Surg 105:e48-e60 |
| Bhagwat, Anand S; Lu, Bin; Vakoc, Christopher R (2018) Enhancer dysfunction in leukemia. Blood 131:1795-1804 |
| Khan, Jalal A; Maki, Robert G; Ravi, Vinod (2018) Pathologic Angiogenesis of Malignant Vascular Sarcomas: Implications for Treatment. J Clin Oncol 36:194-201 |
| Chen, Wei-Chia; Tareen, Ammar; Kinney, Justin B (2018) Density Estimation on Small Data Sets. Phys Rev Lett 121:160605 |
| Cheng, Derek; Tuveson, David (2018) Kras in Organoids. Cold Spring Harb Perspect Med 8: |
| Albrengues, Jean; Shields, Mario A; Ng, David et al. (2018) Neutrophil extracellular traps produced during inflammation awaken dormant cancer cells in mice. Science 361: |
| Cook, Natalie; Basu, Bristi; Smith, Donna-Michelle et al. (2018) A phase I trial of the ?-secretase inhibitor MK-0752 in combination with gemcitabine in patients with pancreatic ductal adenocarcinoma. Br J Cancer 118:793-801 |
| Melnikov, Sergey V; Rivera, Keith D; Ostapenko, Denis et al. (2018) Error-prone protein synthesis in parasites with the smallest eukaryotic genome. Proc Natl Acad Sci U S A 115:E6245-E6253 |
| Krishnan, Navasona; Bonham, Christopher A; Rus, Ioana A et al. (2018) Harnessing insulin- and leptin-induced oxidation of PTP1B for therapeutic development. Nat Commun 9:283 |
| Pommier, Arnaud; Anaparthy, Naishitha; Memos, Nicoletta et al. (2018) Unresolved endoplasmic reticulum stress engenders immune-resistant, latent pancreatic cancer metastases. Science 360: |
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