The major goal of the Protein Chemistry Resource is to provide sequence information of isolated proteins in low abundance at the Cancer Center of Cold Spring Harbor Laboratory. Sequence information is used to isolate genes or identify the genes in the database. Another goal is to analyze post-transcriptional modification of proteins to study protein function, protein-protein interaction and protein-nucleic acid interaction in cancer research. The Resource provides: protein sequence analysis by in-gel digestion, HPLC peptide mapping and automated Edman degradation N-terminal sequencing of protein blotted on PVDF membrane post-translational modification analysis by MALDI-TOF-mass spectrometry and HPLC peptide mapping followed by automated Edman degradation epitope mapping by using mass spectrometry, HPLC and peptide sequencer molecular weight determination of peptides, proteins, and other molecules mass spectrometry sequencing y LCQ deca electrospray mass spectrometry including post-translation modification site analysis Since there are so many proteins isolated with biological significance in all areas of molecular biology, genetics, and cell biology at the Cancer Center, the Protein Chemistry Shared Resource is used by a large number of the Cancer Center at Cold Spring Harbor Laboratory
| Krishnan, Navasona; Konidaris, Konstantis F; Gasser, Gilles et al. (2018) A potent, selective, and orally bioavailable inhibitor of the protein-tyrosine phosphatase PTP1B improves insulin and leptin signaling in animal models. J Biol Chem 293:1517-1525 |
| Borges, Filipe; Parent, Jean-Sébastien; van Ex, Frédéric et al. (2018) Transposon-derived small RNAs triggered by miR845 mediate genome dosage response in Arabidopsis. Nat Genet 50:186-192 |
| Chen, Xiaoyin; Sun, Yu-Chi; Church, George M et al. (2018) Efficient in situ barcode sequencing using padlock probe-based BaristaSeq. Nucleic Acids Res 46:e22 |
| Tonelli, Claudia; Chio, Iok In Christine; Tuveson, David A (2018) Transcriptional Regulation by Nrf2. Antioxid Redox Signal 29:1727-1745 |
| Kumar, Vijay; Rosenbaum, Julie; Wang, Zihua et al. (2018) Partial bisulfite conversion for unique template sequencing. Nucleic Acids Res 46:e10 |
| Lee, Je H (2018) Tracing single-cell histories. Science 359:521-522 |
| Alexander, Joan; Kendall, Jude; McIndoo, Jean et al. (2018) Utility of Single-Cell Genomics in Diagnostic Evaluation of Prostate Cancer. Cancer Res 78:348-358 |
| Huang, Yu-Han; Klingbeil, Olaf; He, Xue-Yan et al. (2018) POU2F3 is a master regulator of a tuft cell-like variant of small cell lung cancer. Genes Dev 32:915-928 |
| Tiriac, Hervé; Belleau, Pascal; Engle, Dannielle D et al. (2018) Organoid Profiling Identifies Common Responders to Chemotherapy in Pancreatic Cancer. Cancer Discov 8:1112-1129 |
| Forcier, Talitha L; Ayaz, Andalus; Gill, Manraj S et al. (2018) Measuring cis-regulatory energetics in living cells using allelic manifolds. Elife 7: |
Showing the most recent 10 out of 380 publications
