The primary service of the 2D Gel Shared Resource is the analysis of protein samples on high-resolution two-dimensional gels. The lab is set up to run large, preparative as well as mini-2 D gels. It has the capacity to run 12 large-format, standardized 2D gels per week, which is made possible by specially designed equipment for casting both first dimension and second dimension slab gels. Furthermore, the 3D Gel lab offers several services for the visualization and detection of separated proteins. The daily running of the facility is carried out by a very experienced research technician, Goula Nourjanova, and overseen by Dr. Linda Van Aelst. G. Nourjanova is supported 100% and Dr. Van Aelst 10% from the CSHL Cancer Center. They provide consultations to the user on sample preparations and analysis. In addition, protocols for sample preparations are accessible from the CSHL website. The availability of a standardized 2D Gel facility, which is capable to deliver high-resolution gels with high reproducibility , has contributed greatly to the research of several members, in particular researchers belonging to the Cancer Genetics and Cell Biology programs of the CSHL Cancer Center. It has contributed to the identification and characterization of post-translational modifications of numerous proteins; some of them playing critical roles in cell cycle control, cytoskeletal organization and apoptosis.
| Fang, Han; Huang, Yi-Fei; Radhakrishnan, Aditya et al. (2018) Scikit-ribo Enables Accurate Estimation and Robust Modeling of Translation Dynamics at Codon Resolution. Cell Syst 6:180-191.e4 |
| Lin, Kuan-Ting; Ma, Wai Kit; Scharner, Juergen et al. (2018) A human-specific switch of alternatively spliced AFMID isoforms contributes to TP53 mutations and tumor recurrence in hepatocellular carcinoma. Genome Res : |
| Wolff, Robert A; Wang-Gillam, Andrea; Alvarez, Hector et al. (2018) Dynamic changes during the treatment of pancreatic cancer. Oncotarget 9:14764-14790 |
| Ryan, Niamh M; Lihm, Jayon; Kramer, Melissa et al. (2018) DNA sequence-level analyses reveal potential phenotypic modifiers in a large family with psychiatric disorders. Mol Psychiatry 23:2254-2265 |
| Danko, Charles G; Choate, Lauren A; Marks, Brooke A et al. (2018) Dynamic evolution of regulatory element ensembles in primate CD4+ T cells. Nat Ecol Evol 2:537-548 |
| Ahrens, Sandra; Wu, Melody V; Furlan, Alessandro et al. (2018) A Central Extended Amygdala Circuit That Modulates Anxiety. J Neurosci 38:5567-5583 |
| Zhang, Tao; Wu, Yen-Ching; Mullane, Patrick et al. (2018) FUS Regulates Activity of MicroRNA-Mediated Gene Silencing. Mol Cell 69:787-801.e8 |
| Arun, Gayatri; Diermeier, Sarah D; Spector, David L (2018) Therapeutic Targeting of Long Non-Coding RNAs in Cancer. Trends Mol Med 24:257-277 |
| Giuliano, Christopher J; Lin, Ann; Smith, Joan C et al. (2018) MELK expression correlates with tumor mitotic activity but is not required for cancer growth. Elife 7: |
| Li, Jiahe; Wu, Connie; Wang, Wade et al. (2018) Structurally modulated codelivery of siRNA and Argonaute 2 for enhanced RNA interference. Proc Natl Acad Sci U S A 115:E2696-E2705 |
Showing the most recent 10 out of 380 publications
