Cancer Proteomics Facility (CPF) The Cancer Proteomics Facility (CPF) provides UPCI investigators access to state-of-the-art techniques and expertise for the detection, quantification, and characterization of cancer-related and biologically relevant proteins for basic, translational, and clinical studies.
The Specific Aims of the CPF are to 1) provide expert guidance in the design and implementation of experiments that use modern protein analysis techniques, including statistical and bioinformatics analysis of the proteomic data; 2) collect, store, and optimally analyze proteins in a wide variety of samples including: cells, tissue, and clinically accessible fluids; 3) provide comprehensive protein identification and characterization services to UPCI investigators; 4) provide targeted quantitative protein assays to UPCI investigators; 5) perform unbiased protein profiling analyses (e.g. SILAC, ITRAQ, and Differential MS) of complex biological samples to identify cancer-related proteins; 6) provide training related to use of and access to all instrumentation and techniques used within CPF, and 7) enable investigators, through the development of a cloud based informatics platform, to use state-of-the-art data management, processing, and analysis tools for proteomic and metabolomic studies. During the past grant cycle, the UPCI and University of Pittsburgh School of Medicine mass spectrometry shared resource facilities have merged and new leadership has been hired to direct this service. Mass spectrometry-based proteomics techniques are now supported through a campus-wide Biomedical Mass Spectrometry Center that is housed on the Oakland campus and directed by Nathan Yates, PhD. This centralized shared resource provides UPCI investigators access to a wide array of high performance mass spectrometry techniques. Antibody based assays are supported by the Luminex laboratory in the Hillman Cancer Center that is directed by Anna Lokshin, PhD and specializes in commercial multiplexed bead based platforms. The CPF has assembled a broad and rapidly advancing set of techniques that allow each researcher to study cancer-related proteins with unmatched sensitivity and specificity. Together, the components of the CPF provide UPCI investigators access to new and specialized techniques for characterizing proteins in cell based animal and patient samples. As a new and growing component of many UPCI research initiatives, the CPF enables investigators to apply mass spectrometry-based, as well as Luminex bead-based, protein measurement techniques to the study of human cells, preclinical models, tumors, and other clinically accessible samples. During the current project period investigators in 9 UPCI Research Programs used the CPF.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA047904-31
Application #
9753959
Study Section
Subcommittee I - Transistion to Independence (NCI)
Project Start
Project End
Budget Start
2019-08-01
Budget End
2020-07-31
Support Year
31
Fiscal Year
2019
Total Cost
Indirect Cost
Name
University of Pittsburgh
Department
Type
DUNS #
004514360
City
Pittsburgh
State
PA
Country
United States
Zip Code
15260
Wang, Yue; Wang, Zehua; Xu, Jieni et al. (2018) Systematic identification of non-coding pharmacogenomic landscape in cancer. Nat Commun 9:3192
Lee, Young-Sun; Lee, Dae-Hee; Choudry, Haroon A et al. (2018) Ferroptosis-Induced Endoplasmic Reticulum Stress: Cross-talk between Ferroptosis and Apoptosis. Mol Cancer Res 16:1073-1076
Tong, Jingshan; Zheng, Xingnan; Tan, Xiao et al. (2018) Mcl-1 Phosphorylation without Degradation Mediates Sensitivity to HDAC Inhibitors by Liberating BH3-Only Proteins. Cancer Res 78:4704-4715
Menk, Ashley V; Scharping, Nicole E; Rivadeneira, Dayana B et al. (2018) 4-1BB costimulation induces T cell mitochondrial function and biogenesis enabling cancer immunotherapeutic responses. J Exp Med 215:1091-1100
Caves, Elizabeth A; Cook, Sarah A; Lee, Nara et al. (2018) Air-Liquid Interface Method To Study Epstein-Barr Virus Pathogenesis in Nasopharyngeal Epithelial Cells. mSphere 3:
Saydmohammed, Manush; Vollmer, Laura L; Onuoha, Ezenwa O et al. (2018) A High-Content Screen Reveals New Small-Molecule Enhancers of Ras/Mapk Signaling as Probes for Zebrafish Heart Development. Molecules 23:
Gough, Albert; Shun, Tong Ying; Taylor, D Lansing et al. (2018) Integrating Analysis of Cellular Heterogeneity in High-Content Dose-Response Studies. Methods Mol Biol 1745:25-46
Fletcher, Rochelle; Wang, Yi-Jun; Schoen, Robert E et al. (2018) Colorectal cancer prevention: Immune modulation taking the stage. Biochim Biophys Acta Rev Cancer 1869:138-148
Li, Xiang; George, Subin M; Vernetti, Lawrence et al. (2018) A glass-based, continuously zonated and vascularized human liver acinus microphysiological system (vLAMPS) designed for experimental modeling of diseases and ADME/TOX. Lab Chip 18:2614-2631
Singh, Renu; Mehrotra, Shailly; Gopalakrishnan, Mathangi et al. (2018) Population pharmacokinetics and exposure-response assessment of veliparib co-administered with temozolomide in patients with myeloid leukemias. Cancer Chemother Pharmacol :

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