Abstract

The primary functions of this core facility are to manage the cancer cell line repository and to assist cancer investigators with cell culture based research. The facility provides shared tissue culture areas which are well equipped and stocked with all of the necessary tissue culture reagents and supplies. Cancer cell lines are cultured and made available to investigators as either growing or frozen cell stocks. Frozen cell stocks are replenished as required. The cancer cell line repository, which has a capacity of 48,000 vials of cells, is managed through a complete inventory system and a computer database. A number of tissue culture related services are offered, including mycoplasma testing, helper virus rescue assays, charcoal stripping of calf serum, and growth curve analysis of new lots of fetal calf serum and calf serum. The genetic labeling of cancer cells with lacZ by either transduction with a retroviral vector or transfection with a plasmid vector, the production of conditioned media from cancer cells and the production of large numbers of cancer cells for animal experiments are developing services. The shared tissue culture areas are utilized by 47 investigators, trainees and technicians from 10 different cancer research laboratories representing CCSG programs. These researchers generally do not have tissue culture space in their individual laboratories. The cancer cell line repository is used by 70 individuals from 17 different cancer research laboratories. Mycoplasma testing is regularly requested by all of these laboratories and by many LCRC member laboratories located elsewhere in the University. The helper virus rescue assay is a recently offered service and has been used by 3 different cancer research laboratories. Genetic labeling of cells with lacZ has been requested by 6 cancer research laboratories, conditioned media production has been requested by 5 cancer research laboratories, and bulk cancer cell production has been requested by 8 cancer research laboratories.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA051008-06
Application #
3731009
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
1995
Total Cost
Indirect Cost

  Institution

Name
Georgetown University
Department
Type
DUNS #
049515844
City
Washington
State
DC
Country
United States
Zip Code
20057

  Publications

Taylor, Teletia R; Dash, Chiranjeev; Sheppard, Vanessa et al. (2018) The effect of a randomized controlled physical activity trial on health related quality of life in metabolically unhealthy African-American women: FIERCE STUDY. Contemp Clin Trials 67:121-128
Kim, Sang-Soo; Rait, Antonina; Garrido-Sanabria, Emilio R et al. (2018) Nanotherapeutics for Gene Modulation that Prevents Apoptosis in the Brain and Fatal Neuroinflammation. Mol Ther 26:84-94
Wallington, Sherrie Flynt; Oppong, Bridget; Iddirisu, Marquita et al. (2018) Developing a Mass Media Campaign to Promote Mammography Awareness in African American Women in the Nation's Capital. J Community Health 43:633-638
O'Neill, Suzanne C; Evans, Chalanda; Hamilton, Rebekah J et al. (2018) Information and support needs of young women regarding breast cancer risk and genetic testing: adapting effective interventions for a novel population. Fam Cancer 17:351-360
Ahn, Jaeil; Conkright, Brian; Boca, Simina M et al. (2018) POPSTR: Inference of Admixed Population Structure Based on Single-Nucleotide Polymorphisms and Copy Number Variations. J Comput Biol 25:417-429
Pannkuk, Evan L; Laiakis, Evagelia C; Garcia, Melissa et al. (2018) Nonhuman Primates with Acute Radiation Syndrome: Results from a Global Serum Metabolomics Study after 7.2 Gy Total-Body Irradiation. Radiat Res :
Chang, Joanne T; Levy, David T; Meza, Rafael (2018) Examining the Transitions Between Cigarette and Smokeless Tobacco Product Use in the United States Using the 2002-2003 and 2010-2011 Longitudinal Cohorts. Nicotine Tob Res 20:1412-1416
Ujjani, Chaitra; Wang, Hongkun; Skarbnik, Alan et al. (2018) A phase 1 study of lenalidomide and ibrutinib in combination with rituximab in relapsed and refractory CLL. Blood Adv 2:762-768
Alamri, Ahmad M; Liu, Xuefeng; Blancato, Jan K et al. (2018) Expanding primary cells from mucoepidermoid and other salivary gland neoplasms for genetic and chemosensitivity testing. Dis Model Mech 11:
Naab, Tammey J; Gautam, Anita; Ricks-Santi, Luisel et al. (2018) MYC amplification in subtypes of breast cancers in African American women. BMC Cancer 18:274

Showing the most recent 10 out of 1120 publications