The Sidney Kimmel Cancer Center Flow Cytometry Shared Resource (FCSR) provides state-of-the-art fluorescence activated cell sorting and analysis capabilities to investigators of the Sidney Kimmel Cancer Center and the Philadelphia region. The most commonly used applications are multi-color cell surface phenotyping, detection of intracellular cytokines and signaling molecules, transfection measurements, studies of apoptosis, cell cycle analysis, cell proliferation, and real time analysis of Ca2+ mobilization kinetics. The sorters, analyzers, and workstations allow for affordable, reliable, and accurate characterization of a wide variety of biological samples. These services provided can be tailored to suit experimental designs in a variety of fields. FCSR provides consultation on current protocols, analysis techniques, and data presentation. This resource plays a critical role in assisting SKCC members performing various research projects directly related to the understanding of the biology and treatment of cancer. Current goals of the facility include: 1) Provide high-quality and affordably priced services for analytical cytometry data acquisition and cytometric cell sorting of various types of biological particles; 2) Consult with SKCC members to determine analytical cytometry needs, aid in the development of appropriate protocols and techniques, and provide technical support for analysis including data analysis and interpretation and/or training in using BD FACS DIVA and FlowJo softwares; 3) Provide training to SKCC members for 24/7 use of automated analytical flow cytometry instrumentation for competent and efficient routine analysis.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA056036-21
Application #
9956990
Study Section
Special Emphasis Panel (ZCA1)
Project Start
Project End
Budget Start
2020-06-01
Budget End
2021-05-31
Support Year
21
Fiscal Year
2020
Total Cost
Indirect Cost
Name
Thomas Jefferson University
Department
Type
DUNS #
053284659
City
Philadelphia
State
PA
Country
United States
Zip Code
19107
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