The High Throughput Analysis (HTA) Laboratory was a developmental facility in the past CCSG renewal, in which it was referred to as the Diagnostics and Therapeutics Screening Core. It is located on the Evanston Campus. The HTA Laboratory's mission is to provide Lurie Cancer Center researchers with advanced technology and expertise for large scale experiments that elucidate the basic biology of cancer and discover novel therapeutic agents. Such experiments can involve parallel manipulation of thousands of samples and the gathering and analysis of data at a similar scale. They include cell-based screening of large compound libraries, massively parallel investigation of gene function using RNAi and gene disruption collections, and high throughput biochemical assays. The facility offers advanced technologies for automated liquid handling, strain collection manipulation, and data acquisition. It provides access to 17 major instruments. These include four advanced platforms for robotic liquid handling, two of which specialize in highly accurate nanoliter liquid dispensing. The facility's analytical equipment includes four photometric plate readers that provide facility users all major detection modes, including simultaneous whole-plate fluorescence measurement for parallel analysis of ion currents and other kinetic phenomena. Additionally, the facility offers access to a Cellomics Arrayscan high-content screening system. The HTA Laboratory integrates these platforms with laboratory infrastructure such as tissue culture, wet bench, and microbial growth chambers, allowing complex in-house work flows. Together these make a broad spectrum of molecular and cell-based assays possible. Importantly, In addition to training and access to instruments, the core offers extensive service for design, validation, and execution of diverse large-scale experiments, including compound library screening and genome-scale functional analysis. The facility also houses biological and chemical libraries for distribution or screening, including a collection of bacterial stocks of snRNAmir lentiviral vector constructs that target nearly all genes in the human and mouse genomes. This capability includes a recently installed tube-based chemical library storage system The HTA Laboratory currently has 251 active users from 76 research groups. Over the last funding period, 79 to 95% of instrument was by Cancer Center member groups. Thus, in its developmental period the facility has become an important established resource for the cancer research community at Northwestern University.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
4P30CA060553-22
Application #
9110829
Study Section
Subcommittee I - Transistion to Independence (NCI)
Project Start
Project End
Budget Start
2016-08-01
Budget End
2017-07-31
Support Year
22
Fiscal Year
2016
Total Cost
Indirect Cost
Name
Northwestern University at Chicago
Department
Type
DUNS #
005436803
City
Chicago
State
IL
Country
United States
Zip Code
60611
Ritzert, Jeremy T; Lathem, Wyndham W (2018) Depletion of Glucose Activates Catabolite Repression during Pneumonic Plague. J Bacteriol 200:
Bui, Triet M; Mascarenhas, Lorraine A; Sumagin, Ronen (2018) Extracellular vesicles regulate immune responses and cellular function in intestinal inflammation and repair. Tissue Barriers 6:e1431038
Park, Yun Ji; Kenney, Grace E; Schachner, Luis F et al. (2018) Repurposed HisC Aminotransferases Complete the Biosynthesis of Some Methanobactins. Biochemistry 57:3515-3523
Forte, Eleonora; Swaminathan, Suchitra; Schroeder, Mark W et al. (2018) Tumor Necrosis Factor Alpha Induces Reactivation of Human Cytomegalovirus Independently of Myeloid Cell Differentiation following Posttranscriptional Establishment of Latency. MBio 9:
Mandelin 2nd, Arthur M; Homan, Philip J; Shaffer, Alexander M et al. (2018) Transcriptional Profiling of Synovial Macrophages Using Minimally Invasive Ultrasound-Guided Synovial Biopsies in Rheumatoid Arthritis. Arthritis Rheumatol 70:841-854
Fong, Lam-Kiu; Wang, Ziwei; Schatz, George C et al. (2018) The Role of Structural Enthalpy in Spherical Nucleic Acid Hybridization. J Am Chem Soc 140:6226-6230
Suraneni, Praveen K; Corey, Seth J; Hession, Michael J et al. (2018) Dynamins 2 and 3 control the migration of human megakaryocytes by regulating CXCR4 surface expression and ITGB1 activity. Blood Adv 2:3540-3552
Yan, M; Lewis, P L; Shah, R N (2018) Tailoring nanostructure and bioactivity of 3D-printable hydrogels with self-assemble peptides amphiphile (PA) for promoting bile duct formation. Biofabrication 10:035010
Edelbrock, Alexandra N; Àlvarez, Zaida; Simkin, Dina et al. (2018) Supramolecular Nanostructure Activates TrkB Receptor Signaling of Neuronal Cells by Mimicking Brain-Derived Neurotrophic Factor. Nano Lett 18:6237-6247
Sullivan, David P; Bui, Triet; Muller, William A et al. (2018) In vivo imaging reveals unique neutrophil transendothelial migration patterns in inflamed intestines. Mucosal Immunol 11:1571-1581

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