Abstract

The Molecular Recognition and Screening Shared Resource (MRSSR) provides VICC investigators with reagents, equipment, and expertise for the production, detection, and characterization of biomolecules. A major role for the MRSSR is to generate monoclonal and phage-displayed single-chain (ScFv) antibodies and to assist investigators in their use in a range of applications. Shared Resource staff also assist in the expression of protein antigens via insect cell expression, scale-up of monoclonal antibody production by growth of hybridomas, and scale-up of ScFvs by expression in E. coli. The insect cell culture facility is also used for high capacity protein expression for biophysical studies and drug screening. Beginning in early 2004, MRSSR staff will assist investigators in the performance of high throughput screens of a large chemical library for activities in vitro or in cell-based assays. Dr. Ray Mernaugh oversees the daily operations of the MRSSR. He has extensive experience in monoclonal and phage-displayed antibody generation as well as protein expression. He meets with users, trains MRSSR personnel, and oversees and coordinates the MSSR's budget, equipment, and use of facilities and services. He is assisted by a highly-trained and experienced staff who are able to help VICC investigators.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA068485-09
Application #
6990167
Study Section
Subcommittee G - Education (NCI)
Project Start
2004-09-28
Project End
2009-08-31
Budget Start
2004-09-28
Budget End
2005-08-31
Support Year
9
Fiscal Year
2004
Total Cost
$120,827
Indirect Cost

  Institution

Name
Vanderbilt University Medical Center
Department
Type
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212

  Publications

Pannala, Venkat R; Wall, Martha L; Estes, Shanea K et al. (2018) Metabolic network-based predictions of toxicant-induced metabolite changes in the laboratory rat. Sci Rep 8:11678
Zhao, Shilin; Li, Chung-I; Guo, Yan et al. (2018) RnaSeqSampleSize: real data based sample size estimation for RNA sequencing. BMC Bioinformatics 19:191
Croessmann, Sarah; Sheehan, Jonathan H; Lee, Kyung-Min et al. (2018) PIK3CA C2 Domain Deletions Hyperactivate Phosphoinositide 3-kinase (PI3K), Generate Oncogene Dependence, and Are Exquisitely Sensitive to PI3K? Inhibitors. Clin Cancer Res 24:1426-1435
Doxie, Deon B; Greenplate, Allison R; Gandelman, Jocelyn S et al. (2018) BRAF and MEK inhibitor therapy eliminates Nestin-expressing melanoma cells in human tumors. Pigment Cell Melanoma Res 31:708-719
Salisbury-Ruf, Christi T; Bertram, Clinton C; Vergeade, Aurelia et al. (2018) Bid maintains mitochondrial cristae structure and function and protects against cardiac disease in an integrative genomics study. Elife 7:
Laroumanie, Fanny; Korneva, Arina; Bersi, Matthew R et al. (2018) LNK deficiency promotes acute aortic dissection and rupture. JCI Insight 3:
Burns, Michael C; Howes, Jennifer E; Sun, Qi et al. (2018) High-throughput screening identifies small molecules that bind to the RAS:SOS:RAS complex and perturb RAS signaling. Anal Biochem 548:44-52
Phelps, Hannah M; Al-Jadiry, Mazin F; Corbitt, Natasha M et al. (2018) Molecular and epidemiologic characterization of Wilms tumor from Baghdad, Iraq. World J Pediatr 14:585-593
Liu, Qi; Herring, Charles A; Sheng, Quanhu et al. (2018) Quantitative assessment of cell population diversity in single-cell landscapes. PLoS Biol 16:e2006687
Almodovar, Karinna; Iams, Wade T; Meador, Catherine B et al. (2018) Longitudinal Cell-Free DNA Analysis in Patients with Small Cell Lung Cancer Reveals Dynamic Insights into Treatment Efficacy and Disease Relapse. J Thorac Oncol 13:112-123

Showing the most recent 10 out of 2462 publications