Abstract

The Molecular Biology Shared Resource seeks to provide the highest quality oligonucleotide synthesis and DNA sequence analysis services in a timely, convenient and cost-effective manner to support the research programs of the Cancer Center. In addition, we provide expert design, data analysis and application troubleshooting assistance. The facility currently operates one Perkin Elmer Biosystems 377 DNA sequencer and two Perkin Elmer 394 oligo synthesizers. During the last three years there has been a substantial increase in use of this shared resource reflecting the quality, convenience and value of the shared provided. Our most striking accomplishment has been the growth in utilization of our DNA sequencing services, increasing from analysis of less than 2400 templates in 1996-1997 to more than 7000 templates in the last year. Utilization of our oligonucleotide synthesis services has also steady grown with an increase from preparation of approximately 2000 oligonucleotides in 1996-1997 to synthesis of almost 2400 oligonucleotides in the last year. We have cared out a niche for this service in the university by combining highest coupling efficiency available with expert assistance with design, convenient ordering methods, and fast turnaround times. Oligonucleotides are ready for the Cancer Center member by the next morning.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA069533-04
Application #
6401790
Study Section
Subcommittee E - Prevention &Control (NCI)
Project Start
1997-08-01
Project End
2005-05-31
Budget Start
Budget End
Support Year
4
Fiscal Year
2000
Total Cost
Indirect Cost

  Institution

Name
Oregon Health and Science University
Department
Type
DUNS #
009584210
City
Portland
State
OR
Country
United States
Zip Code
97239

  Publications

Risom, Tyler; Langer, Ellen M; Chapman, Margaret P et al. (2018) Differentiation-state plasticity is a targetable resistance mechanism in basal-like breast cancer. Nat Commun 9:3815
Minnier, Jessica; Pennock, Nathan D; Guo, Qiuchen et al. (2018) RNA-Seq and Expression Arrays: Selection Guidelines for Genome-Wide Expression Profiling. Methods Mol Biol 1783:7-33
Su, Yulong; Pelz, Carl; Huang, Tao et al. (2018) Post-translational modification localizes MYC to the nuclear pore basket to regulate a subset of target genes involved in cellular responses to environmental signals. Genes Dev 32:1398-1419
Gast, Charles E; Silk, Alain D; Zarour, Luai et al. (2018) Cell fusion potentiates tumor heterogeneity and reveals circulating hybrid cells that correlate with stage and survival. Sci Adv 4:eaat7828
Krey, Jocelyn F; Scheffer, Deborah I; Choi, Dongseok et al. (2018) Mass spectrometry quantitation of proteins from small pools of developing auditory and vestibular cells. Sci Data 5:180128
Rozanov, Dmitri V; Rozanov, Nikita D; Chiotti, Kami E et al. (2018) MHC class I loaded ligands from breast cancer cell lines: A potential HLA-I-typed antigen collection. J Proteomics 176:13-23
Winters-Stone, Kerri M; Wood, Lisa J; Stoyles, Sydnee et al. (2018) The Effects of Resistance Exercise on Biomarkers of Breast Cancer Prognosis: A Pooled Analysis of Three Randomized Trials. Cancer Epidemiol Biomarkers Prev 27:146-153
Pennock, Nathan D; Martinson, Holly A; Guo, Qiuchen et al. (2018) Ibuprofen supports macrophage differentiation, T cell recruitment, and tumor suppression in a model of postpartum breast cancer. J Immunother Cancer 6:98
Xu, Li; Gordon, Ryan; Farmer, Rebecca et al. (2018) Precision therapeutic targeting of human cancer cell motility. Nat Commun 9:2454
Chen, Emerson Y; Blanke, Charles D; Haller, Daniel G et al. (2018) A Phase II Study of Celecoxib With Irinotecan, 5-Fluorouracil, and Leucovorin in Patients With Previously Untreated Advanced or Metastatic Colorectal Cancer. Am J Clin Oncol 41:1193-1198

Showing the most recent 10 out of 277 publications