Abstract

This core facility will generate mutant mice for UCSF Cancer Center investigators. Transgenic mice will be created by microinjecting DNA constructs directly into the pronuclei of fertilized mouse oocytes. Chimeric mice will also be created by microinjecting mouse embryonic stem (ES) cells into 3.5-day old blastocysts. Through these two mechanisms, the Core will facilitate the generation of transgenic, knockout, knock-in and other types of mutant mice for cancer-related research at UCSF. The Core will also perform embryo transfers to allow rederivation of imported and/or infected strains of mice into the specific pathogen-free mouse facility. DNA constructs for microinjection into fertilized oocytes will be purified by the core prior to microinjection using standardized and proven methodology. ES cells will be provided as a freshly-prepared suspension immediately before microinjection. Chimeric and transgenic mice will be born in the core facility colony and then transferred within the UCSF barrier to the relevant investigators? colonies.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA082103-04
Application #
6694324
Study Section
Subcommittee E - Prevention &Control (NCI)
Project Start
2002-09-27
Project End
2007-01-31
Budget Start
Budget End
Support Year
4
Fiscal Year
2002
Total Cost
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Type
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

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Dvorak, Christopher C; Satwani, Prakash; Stieglitz, Elliot et al. (2018) Disease burden and conditioning regimens in ASCT1221, a randomized phase II trial in children with juvenile myelomonocytic leukemia: A Children's Oncology Group study. Pediatr Blood Cancer 65:e27034
Fan, Qi Wen; Nicolaides, Theodore P; Weiss, William A (2018) Inhibiting 4EBP1 in Glioblastoma. Clin Cancer Res 24:14-21
Sannino, Sara; Guerriero, Christopher J; Sabnis, Amit J et al. (2018) Compensatory increases of select proteostasis networks after Hsp70 inhibition in cancer cells. J Cell Sci 131:
Lam, Christine; Ferguson, Ian D; Mariano, Margarette C et al. (2018) Repurposing tofacitinib as an anti-myeloma therapeutic to reverse growth-promoting effects of the bone marrow microenvironment. Haematologica 103:1218-1228
Truillet, Charles; Parker, Matthew F L; Huynh, Loc T et al. (2018) Measuring glucocorticoid receptor expression in vivo with PET. Oncotarget 9:20399-20408
Phillips, Kathryn A; Trosman, Julia R; Deverka, Patricia A et al. (2018) Insurance coverage for genomic tests. Science 360:278-279
Phillips, Kathryn A (2018) Evolving Payer Coverage Policies on Genomic Sequencing Tests: Beginning of the End or End of the Beginning? JAMA 319:2379-2380
Puri, Sapna; Roy, Nilotpal; Russ, Holger A et al. (2018) Replication confers ? cell immaturity. Nat Commun 9:485

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