The Molecular Core will provide several critical services to promote research in drug abuse research. First, we provide a wide range of vector design, construction, and expression services to investigators who need to study the function of a given gene product. This includes epitope-tagged vectors to monitor protein trafficking, or to assess protein-protein interactions. We also provide viral transduction vector design and construction for those investigators who are interested in expression of genes in either primary cells or a cell line. We also provide a wide variety of services for those investigators who are interested in studying the regulation of gene expression. This includes assessment of transcription regulation at the level of gene promoter mapping and function, as well as measurement of mRNA expression by a variety of techniques including quantitative RT-PCR, northern blot analysis, and RNase protection analysis. Finally, we provide genotyping services to the Animal Core, a service which is critical for those strains that must be maintained by breeding heterozygous breeding pairs. In summary, our services are extremely helpful to investigators who are either experienced or inexperienced in molecular biology. It is also apparent that our Core has served to promote interactions and build collaborations between investigators with interests in drug abuse research.

Public Health Relevance

The Molecular Core provides a wide variety of services to investigators interested in drug abuse research, and involved in studies at the molecular level. This Core provides necessary genotyping support for the Animal Core. We provide valuable assistance within Temple University, and to the wider scientific community, and the benefits of our services also include the stimulation of collaborative research efforts.

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Center Core Grants (P30)
Project #
5P30DA013429-12
Application #
8264366
Study Section
Special Emphasis Panel (ZDA1)
Project Start
Project End
Budget Start
2011-05-01
Budget End
2012-04-30
Support Year
12
Fiscal Year
2011
Total Cost
$199,934
Indirect Cost
Name
Temple University
Department
Type
DUNS #
057123192
City
Philadelphia
State
PA
Country
United States
Zip Code
19122
Ward, Sara Jane; Castelli, Francesca; Reichenbach, Zachary W et al. (2018) Surprising outcomes in cannabinoid CB1/CB2 receptor double knockout mice in two models of ischemia. Life Sci 195:1-5
Liu, Jeffrey J; Sharma, Kirti; Zangrandi, Luca et al. (2018) In vivo brain GPCR signaling elucidated by phosphoproteomics. Science 360:
Oliver, Chicora F; Simmons, Steven J; Nayak, Sunil U et al. (2018) Chemokines and 'bath salts': CXCR4 receptor antagonist reduces rewarding and locomotor-stimulant effects of the designer cathinone MDPV in rats. Drug Alcohol Depend 186:75-79
Zewde, Ashenafi Mebratu; Yu, Frances; Nayak, Sunil et al. (2018) PLDT (planarian light/dark test): an invertebrate assay to quantify defensive responding and study anxiety-like effects. J Neurosci Methods 293:284-288
Rom, Slava; Zuluaga-Ramirez, Viviana; Reichenbach, Nancy L et al. (2018) Secoisolariciresinol diglucoside is a blood-brain barrier protective and anti-inflammatory agent: implications for neuroinflammation. J Neuroinflammation 15:25
Ramirez, Servio H; Andrews, Allison M; Paul, Debayon et al. (2018) Extracellular vesicles: mediators and biomarkers of pathology along CNS barriers. Fluids Barriers CNS 15:19
Brailoiu, Eugen; Barlow, Christine L; Ramirez, Servio H et al. (2018) Effects of Platelet-Activating Factor on Brain Microvascular Endothelial Cells. Neuroscience 377:105-113
Barbe, Mary F; Massicotte, Vicky S; Assari, Soroush et al. (2018) Prolonged high force high repetition pulling induces osteocyte apoptosis and trabecular bone loss in distal radius, while low force high repetition pulling induces bone anabolism. Bone 110:267-283
Gentile, Taylor A; Simmons, Steven J; Barker, David J et al. (2018) Suvorexant, an orexin/hypocretin receptor antagonist, attenuates motivational and hedonic properties of cocaine. Addict Biol 23:247-255
Hicks, Callum; Huang, Peng; Ramos, Linnet et al. (2018) Dopamine D1-Like Receptor Agonist and D2-Like Receptor Antagonist (-)-Stepholidine Reduces Reinstatement of Drug-Seeking Behavior for 3,4-Methylenedioxypyrovalerone (MDPV) in Rats. ACS Chem Neurosci 9:1327-1337

Showing the most recent 10 out of 343 publications