Abstract

? Microscopy Imaging and Cellular Physiology Core The Microscopy Imaging and Cellular Physiology (MICP) Core provides members of the Michigan Diabetes Research Center access to state of the art microscopy imaging, in situ hybridization, and electrophysiologic (including optogenetics) analysis, along with expert analysis and support. For over 20 years, the MICPC has provided researchers use of confocal and wide-field microscopes to allow a wide array of cellular and tissue imaging techniques including; imaging of fixed tissues and cells, quantification, co-localization, and live cell imaging including FRET and FRAP experiments. We have now added in situ hybridization analysis with fluorescent or radioisotope or chromogenic substrates for localization and/or quantification of mRNA. The core also has also developed an electrophysiology laboratory that enables the analysis of electrical and ionic changes in neurons, islets, or other relevant tissues, including optogentic activation of these tissues. Core personnel provide extensive expertise in imaging, in situ hybridization and electrophysiology, enabling MDRC investigators to rapidly develop novel experimental ideas and obtain high quality results with expert analysis. Core personnel provide structured service, maintenance and expertise in imaging and cell physiology and related experiments to support diabetes research at Michigan.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK020572-43
Application #
9851844
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2019-12-01
Budget End
2020-11-30
Support Year
43
Fiscal Year
2020
Total Cost
Indirect Cost

  Institution

Name
University of Michigan Ann Arbor
Department
Type
DUNS #
073133571
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109

  Publications

Park, Min-Jung; Iyer, Sapna; Xue, Xiang et al. (2018) HIF1-alpha Regulates Acinar Cell Function and Response to Injury in Mouse Pancreas. Gastroenterology 154:1630-1634.e3
Sucularli, Ceren; Thomas, Peedikayil; Kocak, Hande et al. (2018) High-throughput gene expression analysis identifies p53-dependent and -independent pathways contributing to the adrenocortical dysplasia (acd) phenotype. Gene 679:219-231
Burghardt, Kyle J; Goodrich, Jacyln M; Lines, Brittany N et al. (2018) The Influence of Metabolic Syndrome and Sex on the DNA Methylome in Schizophrenia. Int J Genomics 2018:8076397
Klueh, Ulrike; Ludzinska, Izabela; Czajkowski, Caroline et al. (2018) Crosslinked basement membrane-based coatings enhance glucose sensor function and continuous glucose monitoring in vivo. J Biomed Mater Res A 106:7-16
Ramseyer, Vanesa D; Kimler, Victoria A; Granneman, James G (2018) Vacuolar protein sorting 13C is a novel lipid droplet protein that inhibits lipolysis in brown adipocytes. Mol Metab 7:57-70
Jansson, John-Olov; Palsdottir, Vilborg; Hägg, Daniel A et al. (2018) Body weight homeostat that regulates fat mass independently of leptin in rats and mice. Proc Natl Acad Sci U S A 115:427-432
Xiong, Yi; Torsoni, Adriana Souza; Wu, Feihua et al. (2018) Hepatic NF-kB-inducing kinase (NIK) suppresses mouse liver regeneration in acute and chronic liver diseases. Elife 7:
Djuric, Zora; Bassis, Christine M; Plegue, Melissa A et al. (2018) Colonic Mucosal Bacteria Are Associated with Inter-Individual Variability in Serum Carotenoid Concentrations. J Acad Nutr Diet 118:606-616.e3
Ryan, Karen K; Packard, Amy E B; Larson, Karlton R et al. (2018) Dietary Manipulations That Induce Ketosis Activate the HPA Axis in Male Rats and Mice: A Potential Role for Fibroblast Growth Factor-21. Endocrinology 159:400-413
Liu, Jeffrey M H; Zhang, Xiaomin; Joe, Shelby et al. (2018) Evaluation of biomaterial scaffold delivery of IL-33 as a localized immunomodulatory agent to support cell transplantation in adipose tissue. J Immunol Regen Med 1:1-12

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