Abstract

(taken from application): To provide a central facility for bulk preparation of cell culture media, bacterial media, concentrated reagents for electrophoresis, agar plates and maintenance of specialized cell lines. This central facility has enabled us to enhance quality control, eliminate repetitive services previously performed in individual laboratories, and, by purchasing and preparing media and reagents in bulk quantity, to markedly reduce the cost of experiments using these reagents. The Core also serves as a test site for new lots of serum for the purpose of verifying that a new lot of serum supports growth of a variety of cell types relevant to DERC investigators, thereby ensuring media quality for all investigators. In addition, the Core maintains several specialized antibody-producing cell lines as well as BRL-3A cells which produce MSA [multiplication-stimulating activity]. In collaboration with the Membrane Biology Core, this Core prepares and characterizes bulk quantities of these antibodies and MSA for use by DERC members.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK025295-23
Application #
6564184
Study Section
Project Start
2001-12-01
Project End
2002-11-30
Budget Start
Budget End
Support Year
23
Fiscal Year
2002
Total Cost
$211,312
Indirect Cost

  Institution

Name
University of Iowa
Department
Type
DUNS #
041294109
City
Iowa City
State
IA
Country
United States
Zip Code
52242

  Publications

Harding, Anne Marie S; Kusama, Nobuyoshi; Hattori, Tomonori et al. (2014) ASIC2 subunits facilitate expression at the cell surface and confer regulation by PSD-95. PLoS One 9:e93797
Kusama, Nobuyoshi; Gautam, Mamta; Harding, Anne Marie S et al. (2013) Acid-sensing ion channels (ASICs) are differentially modulated by anions dependent on their subunit composition. Am J Physiol Cell Physiol 304:C89-101
Collier, Daniel M; Peterson, Zerubbabel J; Blokhin, Ilya O et al. (2012) Identification of extracellular domain residues required for epithelial Na+ channel activation by acidic pH. J Biol Chem 287:40907-14
Herlein, Judith A; Fink, Brian D; Henry, Dorlyne M et al. (2011) Mitochondrial superoxide and coenzyme Q in insulin-deficient rats: increased electron leak. Am J Physiol Regul Integr Comp Physiol 301:R1616-24
Herlein, Judy A; Fink, Brian D; Sivitz, William I (2010) Superoxide production by mitochondria of insulin-sensitive tissues: mechanistic differences and effect of early diabetes. Metabolism 59:247-57
Sivitz, William I; Yorek, Mark A (2010) Mitochondrial dysfunction in diabetes: from molecular mechanisms to functional significance and therapeutic opportunities. Antioxid Redox Signal 12:537-77
Elliott, David E; Weinstock, Joel V (2009) Helminthic therapy: using worms to treat immune-mediated disease. Adv Exp Med Biol 666:157-66
Herlein, Judith A; Fink, Brian D; O'Malley, Yunxia et al. (2009) Superoxide and respiratory coupling in mitochondria of insulin-deficient diabetic rats. Endocrinology 150:46-55
Hattori, Tomonori; Chen, Jie; Harding, Anne Marie S et al. (2009) ASIC2a and ASIC3 heteromultimerize to form pH-sensitive channels in mouse cardiac dorsal root ganglia neurons. Circ Res 105:279-86
Fink, Brian D; O'Malley, Yunxia; Dake, Brian L et al. (2009) Mitochondrial targeted coenzyme Q, superoxide, and fuel selectivity in endothelial cells. PLoS One 4:e4250

Showing the most recent 10 out of 690 publications