Abstract

The Epithelial Cell and Mucosal Immunology Core (Core C) provides the infrastructure, biologic resources, expertise, and training for innovative and cross-disciplinary research on epithelial biology and immune function in mucosal surfaces and solid organs of the GI tract. Specific activities and services include: Polarized Epithelial Cell Culture and Genetic Transformations nCounter NanoString RNA Analysis FACs 7- or 12-Color Analysis Of Cultured Cells or Tissue/Organoid-derived Primary Cells Transepithelial Electrophysiology of live cells, cell monolayers, and tissues Gastrointestinal Organoid Derivation and Culture Specialized Equipment, Reagents, Training, and Consultation for recombinant protein expression and purification, including small molecules, specialized and unique epithelial cell lines, expression profiling using Bio-Plex MAGPIX multiplex reader Research and Development of live cell ratio fluorescence imaging of ion and solute transients, and FRAP analysis of membrane molecules A total of 27 HDDC members and 20 non-Members used Core C in the last grant cycle as measured in billable hours of service. 8 HDDC Members used Core C services in every quarter. The Core recorded >4000 billable hours/year. 45 Members and Associate Members anticipate use of Core C in the next grant cycle. All services will be utilized.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK034854-32
Application #
9247866
Study Section
Special Emphasis Panel (ZDK1-GRB-8)
Project Start
Project End
Budget Start
2016-12-01
Budget End
2017-11-30
Support Year
32
Fiscal Year
2017
Total Cost
$178,959
Indirect Cost
$36,303

  Institution

Name
Boston Children's Hospital
Department
Type
Independent Hospitals
DUNS #
076593722
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

McSweeney, Maireade E; Kerr, Jessica; Amirault, Janine et al. (2018) Preoperative Evaluation Is Not Predictive of Transpyloric Feeding Conversion in Gastrostomy-dependent Pediatric Patients. J Pediatr Gastroenterol Nutr 66:887-892
Thiagarajah, Jay R; Kamin, Daniel S; Acra, Sari et al. (2018) Advances in Evaluation of Chronic Diarrhea in Infants. Gastroenterology 154:2045-2059.e6
Masuyer, Geoffrey; Zhang, Sicai; Barkho, Sulyman et al. (2018) Structural characterisation of the catalytic domain of botulinum neurotoxin X - high activity and unique substrate specificity. Sci Rep 8:4518
Xenakis, Jason J; Howard, Emily D; Smith, Kalmia M et al. (2018) Resident intestinal eosinophils constitutively express antigen presentation markers and include two phenotypically distinct subsets of eosinophils. Immunology 154:298-308
Desai, Anal; Alves-Bezerra, Michele; Li, Yingxia et al. (2018) Regulation of fatty acid trafficking in liver by thioesterase superfamily member 1. J Lipid Res 59:368-379
Richmond, Camilla A; Rickner, Hannah; Shah, Manasvi S et al. (2018) JAK/STAT-1 Signaling Is Required for Reserve Intestinal Stem Cell Activation during Intestinal Regeneration Following Acute Inflammation. Stem Cell Reports 10:17-26
Rankin, Carl Robert; Theodorou, Evangelos; Man Law, Ivy Ka et al. (2018) Identification of novel mRNAs and lncRNAs associated with mouse experimental colitis and human inflammatory bowel disease. Am J Physiol Gastrointest Liver Physiol 315:G722-G733
Garcia-Castillo, Maria Daniela; Lencer, Wayne I; Chinnapen, Daniel J-F (2018) Transcytosis Assay for Transport of Glycosphingolipids across MDCK-II Cells. Bio Protoc 8:
Richardson, Gavin David; Sage, Andrew; Bennaceur, Karim et al. (2018) Telomerase Mediates Lymphocyte Proliferation but Not the Atherosclerosis-Suppressive Potential of Regulatory T-Cells. Arterioscler Thromb Vasc Biol 38:1283-1296
Hong, Shangyu; Song, Wei; Zushin, Peter-James H et al. (2018) Phosphorylation of Beta-3 adrenergic receptor at serine 247 by ERK MAP kinase drives lipolysis in obese adipocytes. Mol Metab 12:25-38

Showing the most recent 10 out of 869 publications