Abstract

The Epithelial Cell and Mucosal Immunology Core (Core C) provides the infrastructure, biologic resources, expertise, and training for innovative and cross-disciplinary research on epithelial biology and immune function in mucosal surfaces and solid organs of the GI tract. Specific activities and services include: Polarized Epithelial Cell Culture and Genetic Transformations nCounter NanoString RNA Analysis FACs 7- or 12-Color Analysis Of Cultured Cells or Tissue/Organoid-derived Primary Cells Transepithelial Electrophysiology of live cells, cell monolayers, and tissues Gastrointestinal Organoid Derivation and Culture Specialized Equipment, Reagents, Training, and Consultation for recombinant protein expression and purification, including small molecules, specialized and unique epithelial cell lines, expression profiling using Bio-Plex MAGPIX multiplex reader Research and Development of live cell ratio fluorescence imaging of ion and solute transients, and FRAP analysis of membrane molecules A total of 27 HDDC members and 20 non-Members used Core C in the last grant cycle as measured in billable hours of service. 8 HDDC Members used Core C services in every quarter. The Core recorded >4000 billable hours/year. 45 Members and Associate Members anticipate use of Core C in the next grant cycle. All services will be utilized.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK034854-35
Application #
9850242
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2019-12-01
Budget End
2020-11-30
Support Year
35
Fiscal Year
2020
Total Cost
Indirect Cost

  Institution

Name
Boston Children's Hospital
Department
Type
DUNS #
076593722
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Long, Jonathan Z; Roche, Alexander M; Berdan, Charles A et al. (2018) Ablation of PM20D1 reveals N-acyl amino acid control of metabolism and nociception. Proc Natl Acad Sci U S A 115:E6937-E6945
Bader, Razan M; Jonas, Maureen M; Mitchell, Paul D et al. (2018) Controlled attenuation parameter: A measure of hepatic steatosis in patients with cystic fibrosis. J Cyst Fibros :
Cho, Jin A; Lee, Ann-Hwee; Platzer, Barbara et al. (2018) Retraction Notice to: The Unfolded Protein Response Element IRE1? Senses Bacterial Proteins Invading the ER to Activate RIG-I and Innate Immune Signaling. Cell Host Microbe 23:571
Silvester, Jocelyn A; Faucher, Elizabeth A; McCarty, Caitlin E et al. (2018) Red Spot Lesions in the Duodenal Bulb are a Highly Specific Endoscopic Sign of Celiac Disease: A Prospective Study. J Pediatr Gastroenterol Nutr :
Ohsaki, Asa; Venturelli, Nicholas; Buccigrosso, Tess M et al. (2018) Maternal IgG immune complexes induce food allergen-specific tolerance in offspring. J Exp Med 215:91-113
Pacheco, Alline R; Lazarus, Jacob E; Sit, Brandon et al. (2018) CRISPR Screen Reveals that EHEC's T3SS and Shiga Toxin Rely on Shared Host Factors for Infection. MBio 9:
Ouahed, Jodie; Gordon, William; Canavan, James B et al. (2018) Mucosal Gene Expression in Pediatric and Adult Patients With Ulcerative Colitis Permits Modeling of Ideal Biopsy Collection Strategy for Transcriptomic Analysis. Inflamm Bowel Dis 24:2565-2578
Kumar, Nitin; Abu Dayyeh, Barham K; Lopez-Nava Breviere, Gontrand et al. (2018) Endoscopic sutured gastroplasty: procedure evolution from first-in-man cases through current technique. Surg Endosc 32:2159-2164
Zhang, Sicai; Lebreton, Francois; Mansfield, Michael J et al. (2018) Identification of a Botulinum Neurotoxin-like Toxin in a Commensal Strain of Enterococcus faecium. Cell Host Microbe 23:169-176.e6
Mina, Amir I; LeClair, Raymond A; LeClair, Katherine B et al. (2018) CalR: A Web-Based Analysis Tool for Indirect Calorimetry Experiments. Cell Metab 28:656-666.e1

Showing the most recent 10 out of 869 publications