Abstract

The Epithelial Cell and Mucosal Immunology Core (Core C) provides the infrastructure, biologic resources, expertise, and training for innovative and cross-disciplinary research on epithelial biology and immune function in mucosal surfaces and solid organs of the GI tract. Specific activities and services include: Polarized Epithelial Cell Culture and Genetic Transformations nCounter NanoString RNA Analysis FACs 7- or 12-Color Analysis Of Cultured Cells or Tissue/Organoid-derived Primary Cells Transepithelial Electrophysiology of live cells, cell monolayers, and tissues Gastrointestinal Organoid Derivation and Culture Specialized Equipment, Reagents, Training, and Consultation for recombinant protein expression and purification, including small molecules, specialized and unique epithelial cell lines, expression profiling using Bio-Plex MAGPIX multiplex reader Research and Development of live cell ratio fluorescence imaging of ion and solute transients, and FRAP analysis of membrane molecules A total of 27 HDDC members and 20 non-Members used Core C in the last grant cycle as measured in billable hours of service. 8 HDDC Members used Core C services in every quarter. The Core recorded >4000 billable hours/year. 45 Members and Associate Members anticipate use of Core C in the next grant cycle. All services will be utilized.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK034854-35
Application #
9850242
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2019-12-01
Budget End
2020-11-30
Support Year
35
Fiscal Year
2020
Total Cost
Indirect Cost

  Institution

Name
Boston Children's Hospital
Department
Type
DUNS #
076593722
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Hong, Shangyu; Song, Wei; Zushin, Peter-James H et al. (2018) Phosphorylation of Beta-3 adrenergic receptor at serine 247 by ERK MAP kinase drives lipolysis in obese adipocytes. Mol Metab 12:25-38
Aden, Konrad; Tran, Florian; Ito, Go et al. (2018) ATG16L1 orchestrates interleukin-22 signaling in the intestinal epithelium via cGAS-STING. J Exp Med 215:2868-2886
Smillie, Christopher S; Sauk, Jenny; Gevers, Dirk et al. (2018) Strain Tracking Reveals the Determinants of Bacterial Engraftment in the Human Gut Following Fecal Microbiota Transplantation. Cell Host Microbe 23:229-240.e5
Sallis, Benjamin F; Acar, Utkucan; Hawthorne, Kelsey et al. (2018) A Distinct Esophageal mRNA Pattern Identifies Eosinophilic Esophagitis Patients With Food Impactions. Front Immunol 9:2059
Li, Katherine; Strauss, Richard; Ouahed, Jodie et al. (2018) Molecular Comparison of Adult and Pediatric Ulcerative Colitis Indicates Broad Similarity of Molecular Pathways in Disease Tissue. J Pediatr Gastroenterol Nutr 67:45-52
Li, Yang; Fu, Tian-Min; Lu, Alvin et al. (2018) Cryo-EM structures of ASC and NLRC4 CARD filaments reveal a unified mechanism of nucleation and activation of caspase-1. Proc Natl Acad Sci U S A 115:10845-10852
Morris, Hayley T; Fort, Loic; Spence, Heather J et al. (2018) Loss of N-WASP drives early progression in an Apc model of intestinal tumourigenesis. J Pathol 245:337-348
Panchal, Pratik; Budree, Shrish; Scheeler, Alex et al. (2018) Correction to: Scaling Safe Access to Fecal Microbiota Transplantation: Past, Present, and Future. Curr Gastroenterol Rep 20:28
O'Connell, Amy E; Zhou, Fanny; Shah, Manasvi S et al. (2018) Neonatal-Onset Chronic Diarrhea Caused by Homozygous Nonsense WNT2B Mutations. Am J Hum Genet 103:131-137
Allegretti, J R; Allegretti, A S; Phelps, E et al. (2018) Asymptomatic Clostridium difficile carriage rate post-fecal microbiota transplant is low: a prospective clinical and stool assessment. Clin Microbiol Infect 24:780.e1-780.e3

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