This Core facility is organized to provide technical expertise, equipment and personnel to Liver Center Investigators who wish to work with: a) Isolated cell preparations including: hepatocytes, cholangiocytes, endothelial cells, stellate cells and hepatic lymphocytes, primarily from rat and mouse b) Cell culture facilities for short and long term cultures and cell lines c) Isolated liver perfusion preparations for studies utilizing the whole organ d gene expression using Quantitative real time PCR machine and Phosphor-imaging equipment or e) Alter expression of genes in these cells and tissues using is RNA and adenovirus transfection technologies. By centralizing these procedures in a Core facility, investigators are assured of a high degree of quality control and preparations can often be used simultaneously by more then one investigator, e) Services a-c are also established at the Center?s satellite facility at the Mount Desert Island Biological Laboratory for use of alternative models in marine species, primarily the small skate, Raja erinacea.
Showing the most recent 10 out of 763 publications
