Abstract

The long term objectives of the Diabetes Center Protein and Nucleic Acid Sequencing Core is to provide service, instrumentation, and consultation to Diabetes Center in the area of Protein and nucleic acid chemistry. To achieve these long term goals the following specific aims have been proposed: 1. To provide high sensitivity, HPLC-based, amino acid analysis. 2. To provide high sensitivity protein and peptide sequencing. 3. To provide HPLC-based protein and peptide isolation and peptide mapping. 4. To provide DNA and RNA sequencing capability. 5. To provide oligonucleotide synthesis capability. 6. To provide peptide synthesis capability. These core services are provided either as a service using core personnel or as instrumentation that is available for the individual researcher to use. Depending on the nature of the project, the background of the investigator, and the nature of the technology required each of the listed specific aims can be, or will soon be, provided to the Diabetes Center research community.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK038942-02
Application #
3918091
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
University of Virginia
Department
Type
DUNS #
001910777
City
Charlottesville
State
VA
Country
United States
Zip Code
22904

  Publications

Earnhardt, Richard C; Veldhuis, Johannes D; Cornett, Greg et al. (2002) Pathophysiology of hyperinsulinemia following pancreas transplantation: altered pulsatile versus Basal insulin secretion and the role of specific transplant anatomy in dogs. Ann Surg 236:480-90; discussion 490-1
Lampl, M; Birch, L; Picciano, M F et al. (2001) Child factor in measurement dependability. Am J Hum Biol 13:548-57
Straume, M; Johnson, M L; Veldhuis, J D (1998) Statistically accurate estimation of hormone concentrations and associated uncertainties: methodology, validation, and applications. Clin Chem 44:116-23
Pak, Y; Hong, Y; Kim, S et al. (1998) In vivo chiro-inositol metabolism in the rat: a defect in chiro-inositol synthesis from myo-inositol and an increased incorporation of chiro-[3H]inositol into phospholipid in the Goto-Kakizaki (G.K) rat. Mol Cells 8:301-9
Friend, K; Iranmanesh, A; Login, I S et al. (1997) Pyridostigmine treatment selectively amplifies the mass of GH secreted per burst without altering GH burst frequency, half-life, basal GH secretion or the orderliness of GH release. Eur J Endocrinol 137:377-86
Reyes-Fuentes, A; Chavarria, M E; Aguilera, G et al. (1997) Deconvolution analysis of bioassayable LH secretion and half-life in men with idiopathic oligoasthenospermia. Int J Androl 20:118-25
van den Berg, G; Veldhuis, J D; Frolich, M et al. (1996) An amplitude-specific divergence in the pulsatile mode of growth hormone (GH) secretion underlies the gender difference in mean GH concentrations in men and premenopausal women. J Clin Endocrinol Metab 81:2460-7
Zwart, A D; Urban, R J; Odell, W D et al. (1996) Contrasts in the gonadotropin-releasing hormone dose-response relationships for luteinizing hormone, follicle-stimulating hormone and alpha-subunit release in young versus older men: appraisal with high-specificity immunoradiometric assay and deconvolutio Eur J Endocrinol 135:399-406
Booth Jr, R A; Weltman, J Y; Yankov, V I et al. (1996) Mode of pulsatile follicle-stimulating hormone secretion in gonadal hormone-sufficient and -deficient women--a clinical research center study. J Clin Endocrinol Metab 81:3208-14
Veldhuis, J D (1996) Gender differences in secretory activity of the human somatotropic (growth hormone) axis. Eur J Endocrinol 134:287-95

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