Abstract

application) The purpose of the Biochemistry and Biophysics Core Laboratory is to provide experimental facilities and expertise in biochemical and biophysical techniques. This includes: isolation of plasma membranes and intracellular membranes, analysis of neutral lipid and phospholipid composition of these membranes, analysis of phosphatidylinositol metabolism in cells and membranes, assess the static and dynamic rotational mobilities, measurement of excited state in membrane preparations, measure intracellular Ca and pH in suspensions, measure IP3 levels, DAG mass and glycosphingolipids, Western blotting and immunohistochemical techniques (signal transduction elements). In addition the Core will train users in these methodologies, surveillance and quality control of techniques. The services are said to be provided in a cost-effective manner through core resources, given the expense of the equipment needed to perform these sophisticated analyses.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
2P30DK042086-11
Application #
6450019
Study Section
Project Start
1990-01-01
Project End
2005-11-30
Budget Start
Budget End
Support Year
11
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
University of Chicago
Department
Type
DUNS #
225410919
City
Chicago
State
IL
Country
United States
Zip Code
60637

  Publications

Amin, Ruhul; Asplin, John; Jung, Daniel et al. (2018) Reduced active transcellular intestinal oxalate secretion contributes to the pathogenesis of obesity-associated hyperoxaluria. Kidney Int 93:1098-1107
Miyoshi, Jun; Nobutani, Kentaro; Musch, Mark W et al. (2018) Time-, Sex-, and Dose-Dependent Alterations of the Gut Microbiota by Consumption of Dietary Daikenchuto (TU-100). Evid Based Complement Alternat Med 2018:7415975
Lu, Jing; Lu, Lei; Yu, Yueyue et al. (2018) Effects of Intestinal Microbiota on Brain Development in Humanized Gnotobiotic Mice. Sci Rep 8:5443
Meisel, Marlies; Hinterleitner, Reinhard; Pacis, Alain et al. (2018) Microbial signals drive pre-leukaemic myeloproliferation in a Tet2-deficient host. Nature 557:580-584
Chen, Edmund B; Cason, Cori; Gilbert, Jack A et al. (2018) Current State of Knowledge on Implications of Gut Microbiome for Surgical Conditions. J Gastrointest Surg 22:1112-1123
Chew, Justin; Leypunskiy, Eugene; Lin, Jenny et al. (2018) High protein copy number is required to suppress stochasticity in the cyanobacterial circadian clock. Nat Commun 9:3004
Ruderman, Sarah; Eshein, Adam; Valuckaite, Vesta et al. (2018) Early increase in blood supply (EIBS) is associated with tumor risk in the Azoxymethane model of colon cancer. BMC Cancer 18:814
Dugas, Lara R; Lie, Louise; Plange-Rhule, Jacob et al. (2018) Gut microbiota, short chain fatty acids, and obesity across the epidemiologic transition: the METS-Microbiome study protocol. BMC Public Health 18:978
McIntosh, Christine M; Chen, Luqiu; Shaiber, Alon et al. (2018) Gut microbes contribute to variation in solid organ transplant outcomes in mice. Microbiome 6:96
Overstreet, A M; LaTorre, D L; Abernathy-Close, L et al. (2018) The JAK inhibitor ruxolitinib reduces inflammation in an ILC3-independent model of innate immune colitis. Mucosal Immunol 11:1454-1465

Showing the most recent 10 out of 697 publications