Abstract

? Molecular and Cellular Analytical Core The overall theme of the Molecular and Cellular Analytical (MCA) core is to support investigation of human and non-human metabolism from a cellular/molecular perspective. The overall expertise of the PI in obesity, energy balance, inflammation, and maternal-fetal metabolism provides the nucleus of expertise around which this core lab has been formed. The Core provides analysis and consultation on hormone assays, qPCR, mitochondria function, and cell based assays of cytokines, adipokines, and immune cell isolation tailored to the investigators needs. Over the last grant period the Molecular and Cellular Analytical Core has developed expertise in use of the Seahorse XF24-3 extracellular flux analyzer to allow us to interrogate mitochondrial function. The Molecular and Cellular Analytical Core continues to provide a broad array of assays over a variety of tissue types (fat, muscle, liver, brain), fluids (plasma, media, breast milk), and cells allowing for high throughput for >40 investigators studying nutrition and obesity-related disorders. The Molecular and Cellular Analytical Core lab also provides members of the Center with reagents, access to equipment, and exposure to new techniques (Seahorse technology, cytokine analysis, adipocyte isolation) that have helped to expand the quantity and quality of nutrition research in the NORC.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
2P30DK048520-21
Application #
8975480
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2015-08-01
Budget End
2016-07-31
Support Year
21
Fiscal Year
2015
Total Cost
Indirect Cost

  Institution

Name
University of Colorado Denver
Department
Type
DUNS #
041096314
City
Aurora
State
CO
Country
United States
Zip Code
80045

  Publications

Beli, Eleni; Yan, Yuanqing; Moldovan, Leni et al. (2018) Restructuring of the Gut Microbiome by Intermittent Fasting Prevents Retinopathy and Prolongs Survival in db/db Mice. Diabetes 67:1867-1879
Mintz, Michael; Khair, Shanawaj; Grewal, Suman et al. (2018) Longitudinal microbiome analysis of single donor fecal microbiota transplantation in patients with recurrent Clostridium difficile infection and/or ulcerative colitis. PLoS One 13:e0190997
Lee, Dustin M; Battson, Micah L; Jarrell, Dillon K et al. (2018) SGLT2 inhibition via dapagliflozin improves generalized vascular dysfunction and alters the gut microbiota in type 2 diabetic mice. Cardiovasc Diabetol 17:62
Alexeev, Erica E; Lanis, Jordi M; Kao, Daniel J et al. (2018) Microbiota-Derived Indole Metabolites Promote Human and Murine Intestinal Homeostasis through Regulation of Interleukin-10 Receptor. Am J Pathol 188:1183-1194
Hildreth, Kerry L; Ozemek, Cemal; Kohrt, Wendy M et al. (2018) Vascular dysfunction across the stages of the menopausal transition is associated with menopausal symptoms and quality of life. Menopause 25:1011-1019
Beune, Irene M; Bloomfield, Frank H; Ganzevoort, Wessel et al. (2018) Consensus Based Definition of Growth Restriction in the Newborn. J Pediatr 196:71-76.e1
Keller, A C; Knaub, L A; Scalzo, R L et al. (2018) Sepiapterin Improves Vascular Reactivity and Insulin-Stimulated Glucose in Wistar Rats. Oxid Med Cell Longev 2018:7363485
Creasy, Seth A; Rynders, Corey A; Bergouignan, Audrey et al. (2018) Free-Living Responses in Energy Balance to Short-Term Overfeeding in Adults Differing in Propensity for Obesity. Obesity (Silver Spring) 26:696-702
Lanaspa, Miguel A; Kuwabara, Masanari; Andres-Hernando, Ana et al. (2018) High salt intake causes leptin resistance and obesity in mice by stimulating endogenous fructose production and metabolism. Proc Natl Acad Sci U S A 115:3138-3143
Bergman, Bryan C; Perreault, Leigh; Strauss, Allison et al. (2018) Intramuscular triglyceride synthesis: importance in muscle lipid partitioning in humans. Am J Physiol Endocrinol Metab 314:E152-E164

Showing the most recent 10 out of 756 publications