The goal of the Histology and Molecular Pathology Core is to provide a common facility and expertise for the broad spectrum of morphologic studies that may be required for interpretation of studies of various models of kidney disease and their response to interventions. Additional major functions of this Core include providing tissue samples from existing archival specimens from various kidney disease models that may be used for exploration and characterization of expression patterns of various novel target molecules (tissue microarrays). The Core also aims to develop approaches and enhance existing morphologic techniques to improve the ability of center investigators to localize specific molecules in tissue sections, and to interpret their significance based on the spatial localization and relationship to patterns of injury (e.g. mass spectrometry imaging). The principal morphologic techniques provided by the Core include routine histology, including special stains, and selected immunohistochemistry stains and novel RNA scope in situ hydridization. In addition, electron microscopic analysis and selected morphometric techniques, including glomerular size, tubulointerstitial fibrosis, GBM width and foot process effacement are provided. Laser capture microscopic dissection for special studies of selected renal structures is also offered, with mass spectrometry and/or PCR for molecules of interest in the selected regions. Confocal Z section imaging of selected renal structures for detailed spatial analysis can also be performed by the Core. We already have successfully developed mouse and rat kidney tissue array samples from a wide range of experimental models for assessment of key novel molecules and their response to interventions. We have begun development of in situ mass spectroscopy techniques to optimize spatial resolution of mass spectra in kidney tissues, and propose to fully develop this technique for identification of key molecules in kidney diseases. Thus, the Core will continue to offer both state-of-the-art and standard morphologic assessments, together with development of novel approaches to assess specific molecules in tissue sections, and to interpret their significance based on the spatial localization and relationship to patterns of injury.

Public Health Relevance

The Histology and Molecular Pathology Core will provide a common facility and expertise for the broad spectrum of morphologic studies for interpretation of models of kidney disease and response to interventions, tissue samples from kidney disease models for assessment of expression patterns of novel target molecules and develop novel molecular approaches that enhance existing morphologic techniques to assess specific molecules in injured tissue, and to interpret their significance based on the spatial localization and relationship to patterns of injury.!

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
1P30DK114809-01
Application #
9385114
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2017-07-01
Budget End
2018-06-30
Support Year
1
Fiscal Year
2017
Total Cost
Indirect Cost
Name
Vanderbilt University Medical Center
Department
Type
DUNS #
079917897
City
Nashville
State
TN
Country
United States
Zip Code
37232
Chung, Sungjin; Overstreet, Jessica M; Li, Yan et al. (2018) TGF-? promotes fibrosis after severe acute kidney injury by enhancing renal macrophage infiltration. JCI Insight 3:
Wang, Feng; Katagiri, Daisuke; Li, Ke et al. (2018) Assessment of renal fibrosis in murine diabetic nephropathy using quantitative magnetization transfer MRI. Magn Reson Med 80:2655-2669
Li, Yan; Chung, Sungjin; Li, Zhilian et al. (2018) Fatty acid receptor modulator PBI-4050 inhibits kidney fibrosis and improves glycemic control. JCI Insight 3:
Wang, Feng; Takahashi, Keiko; Li, Hua et al. (2018) Assessment of unilateral ureter obstruction with multi-parametric MRI. Magn Reson Med 79:2216-2227
Zhang, Ming-Zhi; Wang, Suwan; Wang, Yinqiu et al. (2018) Renal Medullary Interstitial COX-2 (Cyclooxygenase-2) Is Essential in Preventing Salt-Sensitive Hypertension and Maintaining Renal Inner Medulla/Papilla Structural Integrity. Hypertension 72:1172-1179
Zhu, Lin; Luu, Thao; Emfinger, Christopher H et al. (2018) CETP Inhibition Improves HDL Function but Leads to Fatty Liver and Insulin Resistance in CETP-Expressing Transgenic Mice on a High-Fat Diet. Diabetes 67:2494-2506
Borza, Corina M; Pozzi, Ambra; Plosa, Erin J (2018) Discoidin Domain Receptor 2, a Potential Therapeutic Target in Lung Fibrosis. Am J Respir Cell Mol Biol 59:277-278
Lim, Beom Jin; Yang, Jae Won; Zou, Jun et al. (2017) Tubulointerstitial fibrosis can sensitize the kidney to subsequent glomerular injury. Kidney Int 92:1395-1403