The Case Center for Synchrotron Biosciences (CSB), with state-of-the-art facilities and laboratories at the National Synchrotron Light Source (NSLS) and at Case Western Reserve University School of Medicine (CWRU), is proposing to continue development and operation of a number of novel synchrotron beam lines to support an international clientele of users in the biomedical sciences. The resource currently serves over 550 users at the NSLS through the operation of four synchrotron beamlines that provide state-of-the art equipment, techniques, user support, and training for radiolytic footprinting, x-ray spectroscopy, and macromolecular crystallography experiments. The users come from across the US and from around the world. Three Technology Service Cores are proposed for the P30 Center. These include a footprinting core, based on the world-leading X28C footprinting beamline, which will provide protein, nucleic acid and in vivo footprinting facilities. An x-ray spectroscopy core, based on the X3B beamline, will receive a detector upgrade bringing its capabilities to state-of-the art in the US. The macromolecular crystallography core, particularly the X29 beamline, will expand its world-class productivity. Efficient mechanisms of delivering user service on our synchrotron beamlines will be continued and are contingent on effective training of users by our experienced beamline staff. The P30 proposes the support of 176 service projects across all three cores that are supported by 212 peer-reviewed grants, including 200 from the NIH. The Technology Service Core infrastructure is closely coupled to the needs of over one hundred user groups with peer-reviewed funding in a wide range of biological sciences. Based on specific developmental activities at the beamlines, user staff will enhance the research of the investigators that are presently using the resource's facilities. Pro-active programs of training and dissemination are outlined that will enhance the reach of the resource's programs and attract new users, expanding the research base.

Public Health Relevance

STATEMENT (provided by applicant): This project provides resources for the NIH funded scientific community to support the study of the structure and dynamics of macromolecuies. These studies are critical for understanding the normal biology of all organisms and the molecular effects of disease including the design of drugs to control cellular processes and the understanding of the molecular interactions that mediate the spread of viruses and bacteria.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Center Core Grants (P30)
Project #
5P30EB009998-04
Application #
8323973
Study Section
Special Emphasis Panel (ZEB1-OSR-B (M2))
Program Officer
Liu, Christina
Project Start
2009-09-01
Project End
2014-08-31
Budget Start
2012-09-01
Budget End
2013-08-31
Support Year
4
Fiscal Year
2012
Total Cost
$696,177
Indirect Cost
$174,845
Name
Case Western Reserve University
Department
Genetics
Type
Schools of Medicine
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106
Zou, Mengnan; Gaowei, Mengjia; Zhou, Tianyi et al. (2018) An all-diamond X-ray position and flux monitor using nitrogen-incorporated ultra-nanocrystalline diamond contacts. J Synchrotron Radiat 25:1060-1067
Porter, Nicholas J; Wagner, Florence F; Christianson, David W (2018) Entropy as a Driver of Selectivity for Inhibitor Binding to Histone Deacetylase 6. Biochemistry 57:3916-3924
Choi, Elliot H; Suh, Susie; Sander, Christopher L et al. (2018) Insights into the pathogenesis of dominant retinitis pigmentosa associated with a D477G mutation in RPE65. Hum Mol Genet 27:2225-2243
Mondal, Prasenjit; Pirovano, Paolo; Das, Ankita et al. (2018) Hydrogen Atom Transfer by a High-Valent Nickel-Chloride Complex. J Am Chem Soc 140:1834-1841
Gao, Ang; Vasilyev, Nikita; Luciano, Daniel J et al. (2018) Structural and kinetic insights into stimulation of RppH-dependent RNA degradation by the metabolic enzyme DapF. Nucleic Acids Res 46:6841-6856
Denler, Melissa C; Wijeratne, Gayan B; Rice, Derek B et al. (2018) MnIII-Peroxo adduct supported by a new tetradentate ligand shows acid-sensitive aldehyde deformylation reactivity. Dalton Trans 47:13442-13458
Mbonye, Uri; Wang, Benlian; Gokulrangan, Giridharan et al. (2018) Cyclin-dependent kinase 7 (CDK7)-mediated phosphorylation of the CDK9 activation loop promotes P-TEFb assembly with Tat and proviral HIV reactivation. J Biol Chem 293:10009-10025
Magherusan, Adriana M; Zhou, Ang; Farquhar, Erik R et al. (2018) Mimicking Class?I?b Mn2 -Ribonucleotide Reductase: A MnII2 Complex and Its Reaction with Superoxide. Angew Chem Int Ed Engl 57:918-922
Yang, Jie; Liu, Zhonghua; Wang, Chuanping et al. (2018) Mechanism of gasdermin D recognition by inflammatory caspases and their inhibition by a gasdermin D-derived peptide inhibitor. Proc Natl Acad Sci U S A 115:6792-6797
Huang, Wei; Peng, Yi; Kiselar, Janna et al. (2018) Multidomain architecture of estrogen receptor reveals interfacial cross-talk between its DNA-binding and ligand-binding domains. Nat Commun 9:3520

Showing the most recent 10 out of 589 publications