Abstract

The Molecular Biology Core provides Center investigators with micro molar and submicromolar amounts of highly purified DNA and RNA oligonucleotides. It is directed by Dr. Roger L. McMacken. The facility synthesizes approximately 200 oligonucleotides per month, which is about one half of its theoretical capacity. Computer-assisted analyses of oligonucleotide secondary structure and characteristics are provided with each sample. The core is also responsible for overseeing the use and maintenance of a Fuji phosphorimager and its associated software, and providing instruction on how to operate the instrument and analyze data.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Center Core Grants (P30)
Project #
5P30ES003819-15
Application #
6575659
Study Section
Project Start
2002-04-01
Project End
2003-03-31
Budget Start
Budget End
Support Year
15
Fiscal Year
2002
Total Cost
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Soneja, Sutyajeet I; Tielsch, James M; Khatry, Subarna K et al. (2017) Characterizing Particulate Matter Exfiltration Estimates for Alternative Cookstoves in a Village-Like Household in Rural Nepal. Environ Manage 60:797-808
Williams, D' Ann L; McCormack, Meredith C; Matsui, Elizabeth C et al. (2016) Cow allergen (Bos d2) and endotoxin concentrations are higher in the settled dust of homes proximate to industrial-scale dairy operations. J Expo Sci Environ Epidemiol 26:42-7
Bose, S; Rivera-Mariani, F; Chen, R et al. (2016) Domestic exposure to endotoxin and respiratory morbidity in former smokers with COPD. Indoor Air 26:734-42
Soneja, Sutyajeet I; Tielsch, James M; Khatry, Subarna K et al. (2016) Highlighting Uncertainty and Recommendations for Improvement of Black Carbon Biomass Fuel-Based Emission Inventories in the Indo-Gangetic Plain Region. Curr Environ Health Rep 3:73-80
Kuo, Chin-Chi; Howard, Barbara V; Umans, Jason G et al. (2015) Arsenic Exposure, Arsenic Metabolism, and Incident Diabetes in the Strong Heart Study. Diabetes Care 38:620-7
Techapiesancharoenkij, Nirachara; Fiala, Jeannette L A; Navasumrit, Panida et al. (2015) Sulforaphane, a cancer chemopreventive agent, induces pathways associated with membrane biosynthesis in response to tissue damage by aflatoxin B1. Toxicol Appl Pharmacol 282:52-60
Vaz, Michelle; Rajasekaran, Subbiah; Potteti, Haranatha R et al. (2015) Myeloid-specific Fos-related antigen-1 regulates cigarette smoke-induced lung inflammation, not emphysema, in mice. Am J Respir Cell Mol Biol 53:125-34
Chawanthayatham, Supawadee; Thiantanawat, Apinya; Egner, Patricia A et al. (2015) Prenatal exposure of mice to the human liver carcinogen aflatoxin B1 reveals a critical window of susceptibility to genetic change. Int J Cancer 136:1254-62
Peng, Roger D; Butz, Arlene M; Hackstadt, Amber J et al. (2015) Estimating the health benefit of reducing indoor air pollution in a randomized environmental intervention. J R Stat Soc Ser A Stat Soc 178:425-443
Ojo, Kristen D; Soneja, Sutyajeet I; Scrafford, Carolyn G et al. (2015) Indoor Particulate Matter Concentration, Water Boiling Time, and Fuel Use of Selected Alternative Cookstoves in a Home-Like Setting in Rural Nepal. Int J Environ Res Public Health 12:7558-81

Showing the most recent 10 out of 356 publications