Abstract

The Molecular Biology Core provides Center investigators with micro molar and submicromolar amounts of highly purified DNA and RNA oligonucleotides. It is directed by Dr. Roger L. McMacken. The facility synthesizes approximately 200 oligonucleotides per month, which is about one half of its theoretical capacity. Computer-assisted analyses of oligonucleotide secondary structure and characteristics are provided with each sample. The core is also responsible for overseeing the use and maintenance of a Fuji phosphorimager and its associated software, and providing instruction on how to operate the instrument and analyze data.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Center Core Grants (P30)
Project #
3P30ES003819-15S1
Application #
6587339
Study Section
Project Start
2002-04-15
Project End
2003-03-31
Budget Start
Budget End
Support Year
15
Fiscal Year
2002
Total Cost
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Soneja, Sutyajeet I; Tielsch, James M; Khatry, Subarna K et al. (2017) Characterizing Particulate Matter Exfiltration Estimates for Alternative Cookstoves in a Village-Like Household in Rural Nepal. Environ Manage 60:797-808
Williams, D' Ann L; McCormack, Meredith C; Matsui, Elizabeth C et al. (2016) Cow allergen (Bos d2) and endotoxin concentrations are higher in the settled dust of homes proximate to industrial-scale dairy operations. J Expo Sci Environ Epidemiol 26:42-7
Bose, S; Rivera-Mariani, F; Chen, R et al. (2016) Domestic exposure to endotoxin and respiratory morbidity in former smokers with COPD. Indoor Air 26:734-42
Soneja, Sutyajeet I; Tielsch, James M; Khatry, Subarna K et al. (2016) Highlighting Uncertainty and Recommendations for Improvement of Black Carbon Biomass Fuel-Based Emission Inventories in the Indo-Gangetic Plain Region. Curr Environ Health Rep 3:73-80
Limjunyawong, Nathachit; Craig, John M; Lagassé, H A Daniel et al. (2015) Experimental progressive emphysema in BALB/cJ mice as a model for chronic alveolar destruction in humans. Am J Physiol Lung Cell Mol Physiol 309:L662-76
Nachman, Rebecca M; Fox, Stephen D; Golden, W Christopher et al. (2015) Serial Free Bisphenol A and Bisphenol A Glucuronide Concentrations in Neonates. J Pediatr 167:64-9
Limjunyawong, Nathachit; Kearson, Alexandra; Das, Sandhya et al. (2015) Effect of point sampling density in quantifying mouse lung emphysema. Anat Rec (Hoboken) 298:531-7
Soneja, Sutyajeet I; Tielsch, James M; Curriero, Frank C et al. (2015) Determining particulate matter and black carbon exfiltration estimates for traditional cookstove use in rural Nepalese village households. Environ Sci Technol 49:5555-62
Limjunyawong, Nathachit; Fallica, Jonathan; Horton, Maureen R et al. (2015) Measurement of the pressure-volume curve in mouse lungs. J Vis Exp :52376
Heaney, Christopher D; Kmush, Brittany; Navas-Acien, Ana et al. (2015) Arsenic exposure and hepatitis E virus infection during pregnancy. Environ Res 142:273-80

Showing the most recent 10 out of 356 publications