B3. Molecular Genetics and EpigeneticsThe Molecular Genetics and Epigenetics component of the IHSFC, formerly called Facility Core C:Microarray and Molecular Biology, has been transformed to address the expressed current and future needs ofCenter members for instrumentation and expertise support in the areas of gene expression, epigenetics andfunctional genomics. The goal of this activity is to provide to Center members access to cutting edgetechnologies in the analysis of gene expression and modulation through access to instrumentation, instruction,assistance in methods and training in the analysis of data. We have added the epigenetics analysis to thisiteration of the grant, replacing resources that had been previously directed towards proteomics. This decisionwas made because the Proteomics Core at Johns Hopkins School of Medicine is(www.hopkinsmedicine.org/msf/) now widely available for Center members and we choose not toduplicate these efforts. The objectives of this component of the IHSFC are to: provide state-of-the-art analysis of gene expression and analysis using the Affymetrix platform for mouse(genome 430A 2.0 array), rat (expression 230 2.0) and human (genome U133 Plus 2.0 array) gene chipsthat comprise the global gene profiling for these respective species; provide infrastructure so that an investigator can understand the global gene expression changes modifiedby environmental exposures that are manifested through epigenetic changes; integrate with the Bioinformatics/Biostatistics core for using Bioconductor and other tools for the analysis ofgenetic and epigenetic changes.
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