Abstract

The Transgenics core will have three divisions: molecular biology; developmental biology, and morphology/pathology. The molecular biology division will provide services related to the cloning of genes or DNAs for the construction of transgene vectors for pronuclear injection or for gene targeting, and for genotyping animals generated in the course of transgenic/ knockout production. The developmental biology division will provide services related to the production of transgenic animals, including DNA and ES cell injections, and ES cell culture. The morphology/pathology division will assist with anatomical analyses of transgenic animals, such as histology, in situ hybridization, or immunohistochemistry. There will be two Ph.D. level supervisors and three technical personnel operating these divisions. Faculty oversight will be co-directed by Drs. Piedrahita and Kier. The facility will be located within a barrier animal facility, and equipment is to be provided by the university. The core, at least in the beginning, aims to generate 2-3 transgenic lines per year and 1-2 knockout lines of mice/year. It is projected that this will increase in the future.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Center Core Grants (P30)
Project #
2P30ES009106-05
Application #
6447864
Study Section
Environmental Health Sciences Review Committee (EHS)
Project Start
1998-04-05
Project End
2007-03-31
Budget Start
Budget End
Support Year
5
Fiscal Year
2002
Total Cost
Indirect Cost

  Institution

Name
Texas A&M University
Department
Type
DUNS #
047006379
City
College Station
State
TX
Country
United States
Zip Code
77845

  Publications

Fan, Yang-Yi; Vaz, Frederic M; Chapkin, Robert S (2017) Dietary fat and fiber interactively modulate apoptosis and mitochondrial bioenergetic profiles in mouse colon in a site-specific manner. Eur J Cancer Prev 26:301-308
Safe, Stephen; Jin, Un-Ho; Morpurgo, Benjamin et al. (2016) Nuclear receptor 4A (NR4A) family - orphans no more. J Steroid Biochem Mol Biol 157:48-60
Davidson, Laurie A; Callaway, Evelyn S; Kim, Eunjoo et al. (2015) Targeted Deletion of p53 in Lgr5-Expressing Intestinal Stem Cells Promotes Colon Tumorigenesis in a Preclinical Model of Colitis-Associated Cancer. Cancer Res 75:5392-7
Hedrick, Erik; Crose, Lisa; Linardic, Corinne M et al. (2015) Histone Deacetylase Inhibitors Inhibit Rhabdomyosarcoma by Reactive Oxygen Species-Dependent Targeting of Specificity Protein Transcription Factors. Mol Cancer Ther 14:2143-53
Phillips, Tracie D; Richardson, Molly; Cheng, Yi-Shing Lisa et al. (2015) Mechanistic relationships between hepatic genotoxicity and carcinogenicity in male B6C3F1 mice treated with polycyclic aromatic hydrocarbon mixtures. Arch Toxicol 89:967-77
Feng, Lingfang; Zhang, Yue; Jiang, Mizu et al. (2015) Up-regulation of Gadd45? after exposure to metal nanoparticles: the role of hypoxia inducible factor 1?. Environ Toxicol 30:490-9
Barhoumi, Rola; Mouneimne, Youssef; Chapkin, Robert S et al. (2014) Effects of fatty acids on benzo[a]pyrene uptake and metabolism in human lung adenocarcinoma A549 cells. PLoS One 9:e90908
Bellum, Sairam; Thuett, Kerry A; Bawa, Bhupinder et al. (2013) The effect of methylmercury exposure on behavior and cerebellar granule cell physiology in aged mice. J Appl Toxicol 33:959-69
Williams, E Spencer; Wilson, Emily; Ramos, Kenneth S (2012) NF-?B and matrix-dependent regulation of osteopontin promoter activity in allylamine-activated vascular smooth muscle cells. Oxid Med Cell Longev 2012:496540
Shaikh, Saame Raza; Jolly, Christopher A; Chapkin, Robert S (2012) n-3 Polyunsaturated fatty acids exert immunomodulatory effects on lymphocytes by targeting plasma membrane molecular organization. Mol Aspects Med 33:46-54

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