- MICROSCOPY For over 40 years, the Microscopy and Imaging Core Facility (MICF) has been a module in the Wilmer P30 Core Grant, evolving from providing transmission and scanning electron microscopy to providing diverse, state of the art equipment to vision scientists and their collaborators. Our community of researchers includes nearly 180 hands on users, from 24 investigators, primarily from Ophthalmology, which includes The Translational Tissue Engineering Center (TTEC), the Center for Nanomedicine (CNM), as well as NIH funded investigators in Biomedical Engineering and The Whiting School of Engineering. The Department of Ophthalmology?s Smith Building houses the Ophthalmology, TTEC and CNM research faculty and is the home of the MICF, allowing convenient access to confocal, multiphoton, electron microscopy, high throughput screening, fluorescent activated cell sorting (FACS) and histological equipment to these investigators. Having these resources housed within one location, easily accessible to all, has resulted in a highly productive and collaborative research program, as demonstrated by the extensive number of NIH-funded projects and publications this Core has supported: 174 publications during the past five years, of which 50% are collaborations between investigators. The personnel supported by this module have a combined 70 years? experience in eye research, providing equipment training and maintenance, consultation on experimental design and frequently develop new methodologies in collaboration with the facility users.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
2P30EY001765-44
Application #
9795547
Study Section
Special Emphasis Panel (ZEY1)
Project Start
Project End
Budget Start
2019-09-30
Budget End
2020-07-31
Support Year
44
Fiscal Year
2019
Total Cost
Indirect Cost
Name
Johns Hopkins University
Department
Type
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21205
Park, Tea Soon; Zimmerlin, Ludovic; Evans-Moses, Rebecca et al. (2018) Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency. J Vis Exp :
Hinkle, Jared T; Perepezko, Kate; Bakker, Catherine C et al. (2018) Domain-specific cognitive impairment in non-demented Parkinson's disease psychosis. Int J Geriatr Psychiatry 33:e131-e139
Duncan, Gregg A; Kim, Namho; Colon-Cortes, Yanerys et al. (2018) An Adeno-Associated Viral Vector Capable of Penetrating the Mucus Barrier to Inhaled Gene Therapy. Mol Ther Methods Clin Dev 9:296-304
Long, Da; Kanan, Yogita; Shen, Jikui et al. (2018) VEGF/VEGFR2 blockade does not cause retinal atrophy in AMD-relevant models. JCI Insight 3:
Liu, Melissa M; Cho, Chris; Jefferys, Joan L et al. (2018) Use of Optical Coherence Tomography by Nonexpert Personnel as a Screening Approach for Glaucoma. J Glaucoma 27:64-70
Ebrahimi, Katayoon B; Cano, Marisol; Rhee, John et al. (2018) Oxidative Stress Induces an Interactive Decline in Wnt and Nrf2 Signaling in Degenerating Retinal Pigment Epithelium. Antioxid Redox Signal 29:389-407
Pitha, Ian; Kimball, Elizabeth C; Oglesby, Ericka N et al. (2018) Sustained Dorzolamide Release Prevents Axonal and Retinal Ganglion Cell Loss in a Rat Model of IOP-Glaucoma. Transl Vis Sci Technol 7:13
Sharma, Anjali; Porterfield, Joshua E; Smith, Elizabeth et al. (2018) Effect of mannose targeting of hydroxyl PAMAM dendrimers on cellular and organ biodistribution in a neonatal brain injury model. J Control Release 283:175-189
Kimball, Elizabeth C; Jefferys, Joan L; Pease, Mary E et al. (2018) The effects of age on mitochondria, axonal transport, and axonal degeneration after chronic IOP elevation using a murine ocular explant model. Exp Eye Res 172:78-85
Nesper, Peter L; Lutty, Gerard A; Fawzi, Amani A (2018) RESIDUAL CHOROIDAL VESSELS IN ATROPHY CAN MASQUERADE AS CHOROIDAL NEOVASCULARIZATION ON OPTICAL COHERENCE TOMOGRAPHY ANGIOGRAPHY: Introducing a Clinical and Software Approach. Retina 38:1289-1300

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