This P30 Ophthalmology Core Facility provides ongoing support for NEI-funded Oregon Health and Science University (OHSU) and Casey Eye Institute vision researchers. The four resource cores are: Bioimaging & Confocal Microscopy; Gene Expression & Manipulation; Genetic Models of Ocular Disease & Biostatistics; and Proteomics. These shared resources will provide equipment and personnel otherwise not available to individual researchers working in a wide range of vision-threatening diseases, including cataracts, glaucoma, macular degeneration, diabetic retinopathy, uveitis, pediatric eye disease, the physiology of vision and the genetics of glaucoma, macular degeneration, uveitis and inherited retinal diseases. The Bioimaging & Confocal Microscopy core will support confocal microscopy studies using state-of-the-art instrumentation for identification and high- resolution localization of proteins. In addition, this core will continue to support small animal imaging through maintenance of a Micron IV Retinal Imaging Microscope for in vivo imaging of rodent eyes. The Gene Expression & Manipulation core will provide instrumentation and technical support for a range of molecular methods to identify changes in levels of gene expression and proteins, and for methods by which these responses can be manipulated, such as RNAi silencing, gene overexpression or gene editing by CRISPR/Cas9. The Genetic Models of Ocular Disease & Biostatistics core (formerly Molecular Genetics & Biostatistics) will continue to provide DNA isolation services from patient blood samples, saliva and tissue and provide access for NEI investigators to advanced statistical techniques to ensure use of appropriate methods both in study design and for data analysis. Biostatistical services include analysis of complex gene expression arrays and RNA-seq datasets, large proteomics studies, optical coherence tomography (OCT) and OCT angiography studies and large patient population data sets from bioinformatics and clinical research studies. Two new services will provide (1) genotyping of cell, tissue and biological samples from human, non-human primate, rat, mouse and pig tissues and (2) provide assistance in establishing primary cell cultures from ocular tissues, as well as fibroblasts from patients with ocular disease. Offering these new services will enable functional genotype-phenotype studies for ocular disease, a critical enhancement in the current age of precision medicine. The Proteomics core will provide access to advanced, high-throughput techniques for measuring changes in protein abundance and modification with disease, determining how proteins fold and interact with one another, and how they regulate development. All four cores are highly complementary and, in combination with new programs designed to encourage communication between clinicians and basic scientists, will increase discoveries with greater direct benefit to patients.

Public Health Relevance

OVERALL COMPONENT This is a core grant (?Ophthalmology Core Facility?) to the Oregon Health and Science University (OHSU) Casey Eye Institute to fund four service cores and support vision researchers studying a wide range of blinding diseases. These entail shared, sophisticated instrumentation and expertise that would be unobtainable by individual researchers. These cores consist of (1) high magnification imaging of proteins in tissue samples and live-imaging technologies for small animal models; (2) methods to detect gene expression within tissues and methods to activate or suppress them to understand how they contribute to eye disease; (3) methods that will help investigators discover gene alterations in patient and model samples and cell cultures, as well as sophisticated statistical expertise to analyze complex gene and population data sets; and (4) methods for detecting protein composition in eye tissues and how they are altered in normal and diseased eyes.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
2P30EY010572-26
Application #
10020817
Study Section
Special Emphasis Panel (ZEY1)
Program Officer
Liberman, Ellen S
Project Start
1997-05-01
Project End
2025-08-31
Budget Start
2020-09-01
Budget End
2021-08-31
Support Year
26
Fiscal Year
2020
Total Cost
Indirect Cost
Name
Oregon Health and Science University
Department
Ophthalmology
Type
Schools of Medicine
DUNS #
096997515
City
Portland
State
OR
Country
United States
Zip Code
97239
Camino, Acner; Zhang, Miao; Liu, Liang et al. (2018) Enhanced Quantification of Retinal Perfusion by Improved Discrimination of Blood Flow From Bulk Motion Signal in OCTA. Transl Vis Sci Technol 7:20
Niffenegger, John H; Soltero, Arysol; Niffenegger, James S et al. (2018) Prevalence of Hepatocyte Growth Factor and Autoantibodies to ?-HGF as a New Etiology for Bilateral Diffuse Uveal Melanocytic Proliferation Masquerading as Neovascular Age-Related Macular Degeneration. J Clin Exp Ophthalmol 9:
Yang, Sungjae; Kopplin, Laura J; Rosenbaum, James T (2018) Retinal vasculitis associated with CREST syndrome. Am J Ophthalmol Case Rep 10:185-188
Hribar, Michelle R; Huang, Abigail E; Goldstein, Isaac H et al. (2018) Data-Driven Scheduling for Improving Patient Efficiency in Ophthalmology Clinics. Ophthalmology :
Loh, Allison R; Edmunds, Beth; Peter Campbell, J et al. (2018) Ophthalmic imaging in children: current practice patterns and perceived barriers. J AAPOS 22:223-225.e3
Hagag, Ahmed M; Pechauer, Alex D; Liu, Liang et al. (2018) OCT Angiography Changes in the 3 Parafoveal Retinal Plexuses in Response to Hyperoxia. Ophthalmol Retina 2:329-336
Lu, Yansha; Simonett, Joseph M; Wang, Jie et al. (2018) Evaluation of Automatically Quantified Foveal Avascular Zone Metrics for Diagnosis of Diabetic Retinopathy Using Optical Coherence Tomography Angiography. Invest Ophthalmol Vis Sci 59:2212-2221
Kim, Sang Jin; Port, Alexander D; Swan, Ryan et al. (2018) Retinopathy of prematurity: a review of risk factors and their clinical significance. Surv Ophthalmol 63:618-637
Adamus, Grazyna (2018) Are Anti-Retinal Autoantibodies a Cause or a Consequence of Retinal Degeneration in Autoimmune Retinopathies? Front Immunol 9:765
Watson, Spencer S; Dane, Mark; Chin, Koei et al. (2018) Microenvironment-Mediated Mechanisms of Resistance to HER2 Inhibitors Differ between HER2+ Breast Cancer Subtypes. Cell Syst 6:329-342.e6

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