Abstract

The Histology and Imaging Core is composed of two facilities providing comprehensive imaging services to the Nebraska Center for the Molecular Biology of Neurosensory Systems (NCNS). The UNMC Tissue Sciences Facility (TSF) has been established to provide basic and specialized histology, staining, immunohistochemistry, and imaging services for studies on animal and human tissues. The TSF offers a full range of cost effective services including tissue preparation and fixing, processing, embedding, sectioning, and standard and special stains for paraffin and frozen samples. Additionally, the TSF offers both bright field and fluorescent imaging and whole slide scanning. There are many software packages available through the TSF for image analysis, reconstruction, and deconvolution as well as technical support for these programs. The resources and services at the TSF are available to all NCNS members, all University of Nebraska personnel, as well as other researchers from outside institutions and industrial users. The Integrated Biomedical Imaging Facility (IBIF) of Creighton University School of Medicine (CUSOM) provides advanced imaging capabilities to researchers of the NCNS and CUSOM and other life science researchers. Advanced imaging, which includes confocal microscopy, multi-photon confoci microsocpy, and other imaging services, is an essential componet of mdern life scicne and medical research. The IBIF has provided and continues to provide these services, plus excellent technical support, to NCNS researchers and anticipates assisting many other current and future investigators associated with the award. This facility is therefore essential to developing their future success as investigators.

Public Health Relevance

Life science and medical investigations require the optimum tissue processing and imaging services to enable the discovery of the basic mechanism underiying development and disease processes. These services, ranging from basic histology to the most advanced imaging, will be provide by these two facilites to further the work of NCNS investigators.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Center Core Grants (P30)
Project #
1P30GM110768-01
Application #
8751152
Study Section
Special Emphasis Panel (ZGM1-TWD-C (3C))
Project Start
Project End
Budget Start
2014-09-05
Budget End
2015-08-31
Support Year
1
Fiscal Year
2014
Total Cost
$85,427
Indirect Cost
$16,781

  Institution

Name
University of Nebraska Medical Center
Department
Type
DUNS #
168559177
City
Omaha
State
NE
Country
United States
Zip Code
68198

  Publications

Leiferman, Amy; Shu, Jiang; Grove, Ryan et al. (2018) A diet defined by its content of bovine milk exosomes and their RNA cargos has moderate effects on gene expression, amino acid profiles and grip strength in skeletal muscle in C57BL/6 mice. J Nutr Biochem 59:123-128
Miura, Hiromi; Quadros, Rolen M; Gurumurthy, Channabasavaiah B et al. (2018) Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors. Nat Protoc 13:195-215
Won, Harim I; Schulze, Thomas T; Clement, Emalie J et al. (2018) De novo Assembly of the Burying Beetle Nicrophorus orbicollis (Coleoptera: Silphidae) Transcriptome Across Developmental Stages with Identification of Key Immune Transcripts. J Genomics 6:41-52
Xia, Xiaohuan; Teotia, Pooja; Ahmad, Iqbal (2018) Lin28a regulates neurogliogenesis in mammalian retina through the Igf signaling. Dev Biol 440:113-128
Baranovskiy, Andrey G; Duong, Vincent N; Babayeva, Nigar D et al. (2018) Activity and fidelity of human DNA polymerase ? depend on primer structure. J Biol Chem 293:6824-6843
Booth, Kevin T; Askew, James W; Talebizadeh, Zohreh et al. (2018) Splice-altering variant in COL11A1 as a cause of nonsyndromic hearing loss DFNA37. Genet Med :
Barta, Cody L; Liu, Huizhan; Chen, Lei et al. (2018) RNA-seq transcriptomic analysis of adult zebrafish inner ear hair cells. Sci Data 5:180005
Alsaad, Hassan A; DeKorver, Nicholas W; Mao, Zhihao et al. (2018) In the Telencephalon, GluN2C NMDA Receptor Subunit mRNA is Predominately Expressed in Glial Cells and GluN2D mRNA in Interneurons. Neurochem Res :
Cruz, Eric; Kumar, Sushil; Yuan, Li et al. (2018) Intracellular amyloid beta expression leads to dysregulation of the mitogen-activated protein kinase and bone morphogenetic protein-2 signaling axis. PLoS One 13:e0191696
Liu, Huizhan; Chen, Lei; Giffen, Kimberlee P et al. (2018) Cell-Specific Transcriptome Analysis Shows That Adult Pillar and Deiters' Cells Express Genes Encoding Machinery for Specializations of Cochlear Hair Cells. Front Mol Neurosci 11:356

Showing the most recent 10 out of 72 publications