The overriding purpose of the Functional Genomics Core (FGC) is to provide to the Scripps NeuroAIDS Preclinical Studies (SNAPS) Center Investigators relevant state of the art technologies and assays for gene function analysis. The SNAPS center Investigators recognize there is likely not to be a single mechanistic cause of CNS dysfunction in NeuroAIDS and therefore have evolved combinatorial and multi- disciplinary research approaches to the examination of basic mechanisms in understanding the pathogenesis of HIV infection in the CNS. The FGC aims to compliment and extend the synergy of our research efforts in this multi-disciplinary research setting, to identify and understand the function of key molecular genetic alterations in the CNS in response to viral and/or host response related perturbations relative to the various neuroAIDS models. In effect, the FGC will provide services and support that facilitate the application of functional genomics to advance our understanding of the pathogenesis of neuroAIDS. The overall specific aims of the FGC are: (1) To provide high-density gene expression analysis using in-house constructed DNA microarrays. In addition, support and advice will be given to Investigators concerning the possible application in their studies of commercially available (e.g. Affymetrix and Incyte) microarray services. (2) To provide multi-probe RNase protection assays to study in more specific and quantitative detail the regulation of candidate genes identified by DNA microarray analysis. (3) To perform single and dual label in situ hybridization procedures for the anatomic and cellular localization of expressed candidate genes. (4) To continually enhance the utility, relevance and ensure services remain state of the art, the FGC will undertake to generate and add new gene probes to the existing DNA microarray, RNase protection and in situ hybridization assays. (5) To provide support to CSPAR Investigators in all aspects of functional genomics. (7) To interact closely with the Administrative Core and other research cores and Investigators to fulfill the obligations of the FGC in the education, outreach and collaborative activities of the CSPAR center.

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Center Core Grants (P30)
Project #
5P30MH062261-03
Application #
6663425
Study Section
Special Emphasis Panel (ZMH1)
Project Start
2002-09-19
Project End
2003-08-31
Budget Start
Budget End
Support Year
3
Fiscal Year
2002
Total Cost
Indirect Cost
Name
Scripps Research Institute
Department
Type
DUNS #
City
La Jolla
State
CA
Country
United States
Zip Code
92037
Olson, Katherine E; Bade, Aditya N; Namminga, Krista L et al. (2018) Persistent EcoHIV infection induces nigral degeneration in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-intoxicated mice. J Neurovirol 24:398-410
Hu, Guoku; Liao, Ke; Niu, Fang et al. (2018) Astrocyte EV-Induced lincRNA-Cox2 Regulates Microglial Phagocytosis: Implications for Morphine-Mediated Neurodegeneration. Mol Ther Nucleic Acids 13:450-463
Schutt, Charles R; Gendelman, Howard E; Mosley, R Lee (2018) Tolerogenic bone marrow-derived dendritic cells induce neuroprotective regulatory T cells in a model of Parkinson's disease. Mol Neurodegener 13:26
Sillman, Brady; Bade, Aditya N; Dash, Prasanta K et al. (2018) Creation of a long-acting nanoformulated dolutegravir. Nat Commun 9:443
Domingo-Almenara, Xavier; Montenegro-Burke, J Rafael; Benton, H Paul et al. (2018) Annotation: A Computational Solution for Streamlining Metabolomics Analysis. Anal Chem 90:480-489
Thomas, Midhun B; Gnanadhas, Divya Prakash; Dash, Prasanta K et al. (2018) Modulating cellular autophagy for controlled antiretroviral drug release. Nanomedicine (Lond) 13:2139-2154
Spooner, Rachel K; Wiesman, Alex I; Mills, Mackenzie S et al. (2018) Aberrant oscillatory dynamics during somatosensory processing in HIV-infected adults. Neuroimage Clin 20:85-91
Kiyota, Tomomi; Machhi, Jatin; Lu, Yaman et al. (2018) URMC-099 facilitates amyloid-? clearance in a murine model of Alzheimer's disease. J Neuroinflammation 15:137
Guijas, Carlos; Montenegro-Burke, J Rafael; Warth, Benedikt et al. (2018) Metabolomics activity screening for identifying metabolites that modulate phenotype. Nat Biotechnol 36:316-320
Kevadiya, Bhavesh D; Woldstad, Christopher; Ottemann, Brendan M et al. (2018) Multimodal Theranostic Nanoformulations Permit Magnetic Resonance Bioimaging of Antiretroviral Drug Particle Tissue-Cell Biodistribution. Theranostics 8:256-276

Showing the most recent 10 out of 374 publications