The goal of this application is to establish core facilities to support basic science research at the Massachusetts General Hospital's Interdepartmental Neuroscience Center. The Center consists of faculty from four departments with shared interests in basic and applied neuroscience. The Center's focus is on the etiology, diagnosis and treatment of a variety of neurological disorders including neurological disorders of childhood onset, traumatic/ischemic brain and spinal cord injury, malignant and benign tumor growth, pathophysiology of pain and neurodegenerative diseases. The research base at the Center has grown enormously since the Center's inception in 1988. The growth is accompanied by an equally strong and rapid increase in demand for shared equipment and facilities. In response to this critical shortage, the Center's investigators have raised institutional and external funding to establish some core services. However, those services fall considerably short of the continually rising demand. In response to the NINDS Program Announcement, a detailed survey of the needs of NINDS-funded laboratories was conducted and an evaluation of existing shared equipment and facilities at the Center was undertaken. Based on those evaluations and taking into account the shared needs, technical expertise and strengths of the Center's faculty, the present application seeks to establish the following 4 core facilities: A) Monoclonal antibody production, B) Microscopy and image analysis, C) Vector development and production, and D) Nucleic acid quantitation. An administrative core to support the Center by ensuring adherence to NINDS guidelines, periodically evaluating core utilization and performance is also proposed. The cores will serve the needs of 15 NINDS-funded projects as well as other projects, as per the NINDS Program requirements. The cores will support ongoing research and stimulate development of novel technology by (i) offering service, equipment and expertise not available in individual laboratories and (ii) fostering synergistic interactions among the Center's investigators. The cores do not overlap with or duplicate in any way existing shared facilities. A detailed plan for efficient and equitable dispensation of services is proposed. Institutional support in the form of research space, funding for equipment purchase and personnel salary has been secured. The core facilities program will offer comprehensive and integrated resources to stimulate and strengthen research activity throughout the Neuroscience Center.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Center Core Grants (P30)
Project #
5P30NS045776-05
Application #
7225560
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Program Officer
Miller, Thomas
Project Start
2003-05-01
Project End
2009-03-31
Budget Start
2007-04-01
Budget End
2009-03-31
Support Year
5
Fiscal Year
2007
Total Cost
$807,500
Indirect Cost
Name
Massachusetts General Hospital
Department
Type
DUNS #
073130411
City
Boston
State
MA
Country
United States
Zip Code
02199
Pagnier, Guillaume J; Kastanenka, Ksenia V; Sohn, Miwon et al. (2018) Novel botanical drug DA-9803 prevents deficits in Alzheimer's mouse models. Alzheimers Res Ther 10:11
Jorfi, Mehdi; D'Avanzo, Carla; Kim, Doo Yeon et al. (2018) Three-Dimensional Models of the Human Brain Development and Diseases. Adv Healthc Mater 7:
Landeira, Bruna Soares; Santana, Themis Taynah da Silva; Araújo, Jéssica Alves de Medeiros et al. (2018) Activity-Independent Effects of CREB on Neuronal Survival and Differentiation during Mouse Cerebral Cortex Development. Cereb Cortex 28:538-548
György, Bence; Cruz, Lilian; Yellen, David et al. (2018) Mutant torsinA in the heterozygous DYT1 state compromises HSV propagation in infected neurons and fibroblasts. Sci Rep 8:2324
DeVos, Sarah L; Corjuc, Bianca T; Commins, Caitlin et al. (2018) Tau reduction in the presence of amyloid-? prevents tau pathology and neuronal death in vivo. Brain 141:2194-2212
Teng, Jian; Hejazi, Seyedali; Hiddingh, Lotte et al. (2018) Recycling drug screen repurposes hydroxyurea as a sensitizer of glioblastomas to temozolomide targeting de novo DNA synthesis, irrespective of molecular subtype. Neuro Oncol 20:642-654
Kovalenko, Marina; Milnerwood, Austen; Giordano, James et al. (2018) HttQ111/+ Huntington's Disease Knock-in Mice Exhibit Brain Region-Specific Morphological Changes and Synaptic Dysfunction. J Huntingtons Dis 7:17-33
Jorfi, Mehdi; D'Avanzo, Carla; Tanzi, Rudolph E et al. (2018) Human Neurospheroid Arrays for In Vitro Studies of Alzheimer's Disease. Sci Rep 8:2450
György, Bence; Sage, Cyrille; Indzhykulian, Artur A et al. (2017) Rescue of Hearing by Gene Delivery to Inner-Ear Hair Cells Using Exosome-Associated AAV. Mol Ther 25:379-391
Maesako, Masato; Horlacher, Jana; Zoltowska, Katarzyna M et al. (2017) Pathogenic PS1 phosphorylation at Ser367. Elife 6:

Showing the most recent 10 out of 147 publications