The major benefits from the core are: 1) creation of a repository for all the expression plasmids needed totag proteins for pull down-based protein isolation procedures and for validating putative binding interactions; 2)provision of shuttle vectors needed to produce lentivirus; 3) provision of state of the art, well characterizedlentivirus vectors to qualified investigators. Lentivirus vectors have proved exceptionally useful in providingefficient transduction of quiescent brain cells that have been traditionally resistant to retroviral-mediatedtransduction. Recombinant lentiviruses do not encode viral proteins and have the benefit of low toxicity andimmunogenicity and produce stable proviral integrants. 4) provision of protocols for isolating tagged proteins.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Center Core Grants (P30)
Project #
1P30NS055088-01A2
Application #
7666414
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
2008-07-01
Project End
2013-06-30
Budget Start
2008-08-01
Budget End
2009-07-31
Support Year
1
Fiscal Year
2008
Total Cost
$233,400
Indirect Cost
Name
University of Washington
Department
Type
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
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